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CD36 Rabbit pAb (bs-1100R)  
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50ul/1180.00元
100ul/1980.00元
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產品編號 bs-1100R
英文名稱 CD36 Rabbit pAb
中文名稱 CD36抗體
別    名 Platelet glycoprotein 4; PAS-4; Platelet glycoprotein IV; Glycoprotein IIIb; GPIV; GPIIIB; GP3B; Leukocyte differentiation antigen CD36; PAS IV; Platelet collagen receptor; Fatty acid translocase; FAT; Thrombospondin receptor; CD_antigen: GP4; CD36_HUMAN.  
Specific References  (4)     |     bs-1100R has been referenced in 4 publications.
[IF=4.109] Yu Haiyi. et al. The Foam Cell Formation Associated With Imbalanced Cholesterol Homeostasis Due to Airborne Magnetite Nanoparticles Exposure. TOXICOL SCI. 2022 Aug;:  WB ;  Mouse, Human.  
[IF=3.974] Astragaloside IV inhibits palmitate-mediated oxidative stress and fibrosis in human glomerular mesangial cells via downregulation of CD36 expression Y Su  WB ;  Rat &Human.  
[IF=2.752] Jie Song. et al. Ethanol Extract of Artemisia Annua Prevents LPS-Induced Inflammation and Blood–Milk Barrier Disruption in Bovine Mammary Epithelial Cells. ANIMALS. 2022 Jan;12(10):1228  WB ;  Bovine.  
[IF=0] Afolabi Oladele Ayobami. et al. Diet-induced insulin resistance altered cardiac GLUT4 and FATP/CD36 expression in rats. Beni-Suef University Journal of Basic and Applied Sciences. 2022 Dec;11(1):1-8  IHC ;  Rat.  
研究領域 腫瘤  心血管  細胞生物  免疫學  干細胞  細胞膜受體  細胞類型標志物  血管內皮細胞  內皮細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1ug/Test,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 52 kDa
檢測分子量 85-90
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD36: 301-400/472 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

Function:
Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects.

Subunit:
Interacts with THBS1 and THBS2; the interactions mediate the THBS antiangiogenic activity.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Predominant in heart, intestine, spleen, fat, skeletal muscle, lower in testes.

Post-translational modifications:
N-glycosylated and O-glycosylated with a ratio of 2:1.

DISEASE:
Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].

Similarity:
Belongs to the CD36 family.

SWISS:
P16671

Gene ID:
948

Database links:

Entrez Gene: 948 Human

Entrez Gene: 12491 Mouse

Entrez Gene: 29184 Rat

Omim: 173510 Human

SwissProt: P16671 Human

SwissProt: Q08857 Mouse

SwissProt: Q07969 Rat

Unigene: 120949 Human

Unigene: 18628 Mouse

Unigene: 406799 Mouse

Unigene: 102418 Rat

Unigene: 205840 Rat




CD36屬一種多功能細胞膜受體,CD36參與長鏈脂肪酸的吸附和調節細胞凋亡殘物的清理和巨噬細胞的吞噬作用, 屬于一種抗蛋白酶血小板膜表面糖蛋白。
CD36 廣泛在單核細胞、巨噬細胞、內皮層細胞、血小板、紅細胞前板、脂肪細胞、肌肉細胞以及乳腺內皮層細胞中有不同的表達。CD36抗原是血小板反應素的配體,兩者結合后產生抑制腫瘤血管生成的效應。
產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with CD36 polyclonal antibody, unconjugated (bs-1100R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
The U937(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD36 antibody (bs-1100R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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