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Connexin-40 Rabbit pAb (bs-1050R)  
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產品編號 bs-1050R
英文名稱 Connexin-40 Rabbit pAb
中文名稱 間隙連接蛋白40抗體
別    名 connexin 40; connexin40; CX40; Gap junction alpha 5 protein; gap junction protein alpha 5 40kD(connexin 40); gap junction protein alpha 5; GJA5; MGC11185; CXA5_HUMAN; Gap junction alpha-5 protein; Connexin-40; Cx40.  
Specific References  (3)     |     bs-1050R has been referenced in 3 publications.
[IF=5.9] Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.  WB ;  Dog.  
[IF=3.322] Zhangchi Liu. et al. Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats. BIOCHEM BIOPH RES CO. 2022 Dec;636:33  WB ;  Rat.  
[IF=0] Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40.  WB ;  Rat.  
研究領域 心血管  信號轉導  細胞粘附分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 40 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Connexin-40: 301-358/358 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]

Function:
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subunit:
A connexon is composed of a hexamer of connexins.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.

DISEASE:
Familial atrial standstill (FAS) [MIM:108770]: Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. Note=The disease may be caused by mutations affecting distinct genetic loci, including the gene represented in this entry. A rare GJA5 genotype has been detected in combination with a mutation in SCN5A in a large family with atrial standstill.
Familial atrial fibrillation 11 (ATFB11) [MIM:614049]: A familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the connexin family. Alpha-type (group II) subfamily.

SWISS:
P36382

Gene ID:
2702

Database links:

Entrez Gene: 2702 Human

Omim: 121013 Human

SwissProt: P36382 Human

Unigene: 447968 Human



Cx40間隙連接蛋白40 是構成細胞間的通道,小分子成份可以借此在細胞間擴散。Connexin-40也是心肌縫隙連接的主要蛋白之一。
此外,星形細胞、成纖維細胞、平滑肌和腎等組織也有表達Connexin40。
產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Connexin-40 polyclonal antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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