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GAD65 Rabbit pAb (bs-0325R)  
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產(chǎn)品編號(hào) bs-0325R
英文名稱(chēng) GAD65 Rabbit pAb
中文名稱(chēng) 谷氨酸脫羧酶-65抗體
別    名 65 kDa glutamic acid decarboxylase; DCE 2; DCE2; GAD 2; GAD 65; GAD-2; GAD-65; GAD2; Glutamate Decarboxylase 2(pancreatic islets and brain 65kDa); Glutamate Decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.  
研究領(lǐng)域 免疫學(xué)  糖尿病  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 65 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant mouse GAD65 protein 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Function:
Catalyzes the production of GABA.

Subunit:
Homodimer.

Subcellular Location:
Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.

Post-translational modifications:
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.

Similarity:
Belongs to the group II decarboxylase family.

SWISS:
P48320

Gene ID:
14417

Database links:

Entrez Gene: 2572 Human

Entrez Gene: 14417 Mouse

Entrez Gene: 24380 Rat

SwissProt: Q05329 Human

SwissProt: P48320 Mouse

SwissProt: Q05683 Rat

Unigene: 231829 Human

Unigene: 4784 Mouse

Unigene: 29951 Rat



谷氨酸脫羧酶-65(GAD65)是用于I II型糖尿病研究的很重要的蛋白。
產(chǎn)品圖片
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Mouse Hippocampus tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Cerebellum tissue lysates Lane 6: Rat Hippocampus tissue lysates Lane 7: Human SH-SY5Y cell lysates Lane 8: Human U-87 MG cell lysates Lane 9: Human Hela cell lysates Lane 10: Human Jurkat cell lysates Primary: Anti-GAD65 (bs-0325R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 62 kDa
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