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Nestin Rabbit pAb (bs-0008R)  
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產品編號 bs-0008R
英文名稱 Nestin Rabbit pAb
中文名稱 巢蛋白/神經上皮干細胞蛋白抗體
別    名 ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.  
Specific References  (14)     |     bs-0008R has been referenced in 14 publications.
[IF=10.317] Yu D et al. MOF-encapsulated nanozyme enhanced siRNA combo: Control neural stem cell differentiation and ameliorate cognitive impairments in Alzheimer's disease model. Biomaterials . 2020 Oct;255:120160.  ICF ;  Rat.  
[IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390  IF ;  Mouse.  
[IF=6.081] Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936  ICC ;  Human.  
[IF=5.195] Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684  IF, WB ;  Rat.  
[IF=5.116] Mengna Duan. et al. Epidermal stem cell-derived exosomes promote skin regeneration by downregulating transforming growth factor-β1 in wound healing. Stem Cell Res Ther. 2020 Dec;11(1):1-11  IHC ;  Rat.  
[IF=4.495] Fumiaki Sato. et al. Pulp tissue reaction to a self-adhesive, resin-based direct pulp capping material containing surface pre-reacted glass-ionomer filler. Dent Mater. 2021 Mar;:  IHC ;  Rat.  
[IF=4.26] Wang et al. Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells. (2016) Sci.Re. 6:25946  IF(ICC) ;  Rat.  
[IF=3.17] Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.  IHC-P ;  Mouse.  
[IF=3.144] Kü?üKKAYA EREN Selen. et al. Combined effects of bone morphogenetic protein-7 and mineral trioxide aggregate on the proliferation, migration, and differentiation of human dental pulp stem cells. J APPL ORAL SCI. 2022 Sep;30:  ICC ;  Human.  
[IF=1.918] Yong Du. et al. Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development. Med Sci Monitor. 2020; 26: e927334-1–e927334-10  IHC ;  Mouse.  
[IF=1.64] Shan, Tengfei, et al. "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β‐catenin signaling." Cell Biology International (2014).  IF(ICC) ;  Mouse.  
[IF=1.396] He X et al. Characterization of Tibetan Mastiff pancreatic progenitor cells and differentiation into insulin-secreting cells.  ICF ;  Tibetan Mastiff pancreas.  
[IF=1.18] Ma Caiyun. et al. In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells. BRAZ ARCH BIOL TECHN. 2023 Feb;66:  IF ;  Human.  
[IF=0.97] Kawashima, Satoki, Koichi SHINKAI, and Masaya SUZUKI. "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping." Dental Materials Journal 35.3 (2016): 479-489.  IHC-P ;  Rat.  
研究領域 腫瘤  心血管  發育生物學  神經生物學  干細胞  細胞類型標志物  腫瘤細胞生物標志物  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse (predicted: Rat)
產品應用 Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 209 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from rat Nestin: 681-750/1893 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:
cytoplasm

Tissue Specificity:
CNS stem cells.

Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P21263

Gene ID:
25491

Database links:

Entrez Gene: 10763 Human

Entrez Gene: 18008 Mouse

Entrez Gene: 25491 Rat

Omim: 600915 Human

SwissProt: P48681 Human

SwissProt: Q6P5H2 Mouse

SwissProt: P21263 Rat

Unigene: 527971 Human

Unigene: 331129 Mouse

Unigene: 9701 Rat





神經干細胞標志物


在動物體早期發育過程中,中樞神經系統發育尤為重要 。Nestin是一種中等纖維蛋白,它在哺乳動物神經前體細胞中表達較高,目前已被廣泛用于神經前體細胞的重要標志分子。研究Nestin的表達調控規律,將有助于人們認識神經前體細胞的生物特性。此抗體還表達中樞神經系統干細胞及神經上皮細胞。
產品圖片
Cell: Neuro-2a Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control on Neuro-2a(blue) compared with primary antibody(Cat#: bs-0008R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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