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PLGF Rabbit pAb (bs-0281R)  
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產品編號 bs-0281R
英文名稱 PLGF Rabbit pAb
中文名稱 胎盤生長因子抗體
別    名 Placenta growth factor; PGF; PGFL; PIGF; Placental growth factor vascular endothelial growth factor related protein; PlGF 2; PLGF; PlGF2; PLGF_HUMAN; Vascular endothelial growth factor related protein.  
研究領域 腫瘤  細胞生物  免疫學  生長因子和激素  轉錄調節因子  血管內皮細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PLGF: 166-221/221 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The placental-derived growth factor (PIGF) is a dimeric glycoprotein showing a high degree of sequence similarity to the vascular endothelial growth factor. Alternative splicing of the PIGF primary transcript gives rise to two forms, named PIGF-1 and PIGF-2, which differ only in the insertion of a highly basic 21-amino acid stretch at the carboxyl end. The presence of the PIGF mRNA in thyroid, placenta, lung, and goiter has indicated the tissues where this factor functions. However, the role of PIGF in vascular development has not yet been clearly established.

Function:
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.

Subunit:
Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF. Isoform PlGF-3 is found both as homodimer and as monomer.

Subcellular Location:
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

Tissue Specificity:
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P49763

Gene ID:
5228

Database links:

Entrez Gene: 5228 Human

Entrez Gene: 18654 Mouse

Omim: 601121 Human

SwissProt: P49763 Human

SwissProt: P49764 Mouse

Unigene: 252820 Human

Unigene: 4809 Mouse



PLGF胎盤生長因子是血管生長因子家族成員之一,是動脈粥樣硬化斑塊不穩定的主要炎性激活物,因此它可作為ACS患者風險預測的生物學標志物.尿胎盤生長因子水平可預測先兆子癇危險.研究人員發現,在疾病發作前的6到8周,先兆子癇患者尿液中的胎盤生長因子水平急劇下降,這種新的尿檢方法使提前1到2個月預測先兆子癇成為可能。亦有研究人員正在從事胎盤生長因子與肺氣腫,胃癌,腸癌等方面的研究。
相關抗體 bs-0280R(抗大鼠) bs-0234R(抗小鼠)
產品圖片
Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-PLGF (bs-0281R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach carcer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Placenta growth factor; PLGF) Polyclonal Antibody, Unconjugated (bs-0281R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PLGF Polyclonal Antibody, Unconjugated(bs-0281R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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