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SLC5A1 Rabbit pAb (bs-1128R)  
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產品編號 bs-1128R
英文名稱 SLC5A1 Rabbit pAb
中文名稱 鈉-糖共轉運載體1抗體
別    名 D22S675; High affinity sodium glucose cotransporter 1; GLT1; GLT-1; High affinity sodium glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; NAGT; SGLT 1; SGLT1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5(sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1.  
Specific References  (6)     |     bs-1128R has been referenced in 6 publications.
[IF=5.846] Gai-feng Hu. et al. SGLT1/2 as the potential biomarkers of renal damage under Apoe?/? and chronic stress via the BP neural network model and support vector machine. FRONT CARDIOVASC MED. 2022; 9: 948909  IF ;  Mouse.  
[IF=5.561] Yang Liu. et al. Methylation, Hydroxylation, Glycosylation and Acylation Affect the Transport of Wine Anthocyanins in Caco-2 Cells. FOODS. 2022 Jan;11(23):3793  WB ;  Human.  
[IF=5.318] Yaoyao Liu. et al. Cinnamon free phenolic extract regulates glucose absorption in intestinal cells by inhibiting glucose transporters. FOOD BIOSCI. 2023 Apr;52:102405  WB ;  Human.  
[IF=5.075] Qun Cheng. et al. Zearalenone Exposure Affects the Keap1–Nrf2 Signaling Patway and Glucose Nutrient Absorption Related Genes of Porcine Jejunal Epithelial Cells. TOXINS. 2022 Nov;14(11):793  WB ;  Pig.  
[IF=3.457] Wang N et al. Fibroblast growth factor 21 improves glucose homeostasis partially via down-regulation of Na+-d-glucose cotransporter SGLT1 in the small intestine.(2019) Biomedicine & Pharmacotherapy, 109, 1070–1077.  WB ;  Mouse.  
[IF=2.99] Chen, Zhaolin, et al. "Efficiency of transcellular transport and efflux of flavonoids with different glycosidic units from flavonoids of< i> Litsea coreana L in a MDCK epithelial cell monolayer model." European Journal of Pharmaceutical Sciences (2013).  WB ;  Dog.  
研究領域 神經生物學  轉運蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 73 kDa
檢測分子量
細胞定位 細胞外基質 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SGLT1: 501-600/665 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function:
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed mainly in intestine and kidney.

Post-translational modifications:
N-glycosylation is not necessary for the cotransporter function.

DISEASE:
Congenital glucose/galactose malabsorption (GGM) [MIM:606824]: Intestinal monosaccharide transporter deficiency. It is an autosomal recessive disorder manifesting itself within the first weeks of life. It is characterized by severe diarrhea and dehydration which are usually fatal unless glucose and galactose are eliminated from the diet. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

SWISS:
P13866

Gene ID:
6523

Database links:

Entrez Gene: 6523 Human

Entrez Gene: 20537 Mouse

Entrez Gene: 397113 Pig

Entrez Gene: 25552 Rat

Omim: 182380 Human

SwissProt: P13866 Human

SwissProt: Q8C3K6 Mouse

SwissProt: P53790 Rat

Unigene: 1964 Human

Unigene: 25237 Mouse

Unigene: 10224 Rat



葡萄糖的跨膜轉運主要是通過SGLT1結合1 mol葡萄糖,2 mol的Na+,形成Na+-載體-葡萄糖復合物,順Na+的濃度梯度進入細胞.不同物種的SGLT1具有較高的同源性.
產品圖片
Sample: Lane 1: Mouse Kidney Lysates Lane 2: Human 293T cell Lysates Lane 3: Human HepG2 cell Lysates Primary: Anti-SLC5A1 (bs-1128R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73kDa Observed band size: 70kDa
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (bs-1128R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min Block endogenous peroxidase by 3% Hydrogen peroxide for 30min Blocking buffer (normal goat serum) at 37℃ for 20 min Incubation: Anti-SGLT1 Polyclonal Antibody, Unconjugated(bs-1128R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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