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CD28 Rabbit pAb (bs-1297R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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產品編號 bs-1297R
英文名稱 CD28 Rabbit pAb
中文名稱 CD28抗體
別    名 CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN; CD28_MOUSE.  
Specific References  (1)     |     bs-1297R has been referenced in 1 publications.
[IF=2.06] Liu, Shangjing, et al. ?Costimulatory Molecule CD28 Participates in the Process of Embryo Implantation in Mice.? Reproductive Sciences (2013): 1933719113512537.  IHC-F ;  Mouse.  
研究領域 細胞生物  免疫學  干細胞  細胞粘附分子  細胞表面分子  淋巴細胞  t-淋巴細胞  b-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Pig,Cow,Chicken,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 25 kDa
檢測分子量 45
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD28: 1-120/218 <Extracellular>
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Function:
Possibly involved in T-cell activation.

Subunit:
Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P31041

Gene ID:
12487

Database links:

Entrez Gene: 940 Human

Entrez Gene: 12487 Mouse

Omim: 186760 Human

SwissProt: P10747 Human

SwissProt: P31041 Mouse

SwissProt: P42069 Rabbit

Unigene: 591629 Human

Unigene: 255003 Mouse



CD28是免疫球蛋白超家族成員之一 ,為T細胞特異性由二硫鍵連接的同源二聚體表面糖蛋白,主要分布在外周血淋巴細胞。對遲發型超敏反應以及Tc介導的細胞毒作用都有免疫調節作用。
產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody Antibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody Antibody: Goat anti-rabbit IgG-FITC; Supplier: Cell Signaling Technology; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody Supplier: Bioss; Supplier catalog number: bs-1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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