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RANTES Rabbit pAb (bs-1324R)  
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產(chǎn)品編號(hào) bs-1324R
英文名稱 RANTES Rabbit pAb
中文名稱 T細(xì)胞特異性趨化因子抗體
別    名 RANTES; CCL5; TCP228; Small Inducible Cytokine A5; CCL5; SISd; MGC17164; SCYA5; RANTES; D17S136E; SIS-delta; chemokine(C-C motif) ligand 5; regulated on activation normal T cell expressed and secreted; chemokine(C-C motif) ligand 5; T-cell-specific protein RANTES; C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; RANTES(3-68); RANTES(4-68);  
Specific References  (8)     |     bs-1324R has been referenced in 8 publications.
[IF=9.038] Bing Han. et al. Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway. J Hazard Mater. 2021 May;:125984  WB ;  Mouse.  
[IF=5.38] Pattappa, G., et al. "CCL5/RANTES is a key chemoattractant released by degenerative intervertebral discs in organ culture." European Cells & Materials 27 (2014): 124.  IHC-P ;  Bovine.  
[IF=5.037] Yi Wu. et al. Targeting cIAPs attenuates CCl4-induced liver fibrosis by increasing MMP9 expression derived from neutrophils. Life Sci. 2022 Jan;289:120235  IHC ;  Mouse.  
[IF=4.3] Hwaiz, Rundk, et al. "Rac1-dependent secretion of platelet-derived CCL5 regulates neutrophil recruitment via activation of alveolar macrophages in septic lung injury." Journal of Leukocyte Biology (2015): jlb-4A1214.  Mouse.  
[IF=3.486] Linardi Daniele. et al. Slow versus fast rewarming after hypothermic circulatory arrest: effects on neuroinflammation and cerebral oedema. Eur J Cardio-Thorac. 2020 Oct;58(4):792-800  IHC ;  Rat.  
[IF=2.81] Sun, Yubo, et al. "Biological effects and osteoarthritic disease‐modifying activity of small molecule CM‐01." Journal of Orthopaedic Research (2017).  IHC-P ;  Guinea Pig.  
[IF=1.75] Irie, Koichiro, et al. "Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium." Archives of oral biology (2017).  IF(IHC-P) ;  Mouse.  
[IF=1.74] Sun et al. Disease-modifying effects of phosphocitrate and phosphocitrate-β-ethyl ester on partial meniscectomy-induced osteoarthritis. (2015) BMC.Musculoskelet.Disord. 16:270  IHC ;  Guinea Pig.  
研究領(lǐng)域 腫瘤  免疫學(xué)  趨化因子  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Pig (predicted: Rat,Rabbit,Sheep,Cow,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 7.4/10 kDa
檢測(cè)分子量
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CCL5: 62-91/91 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.

Function:
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.

Subcellular Location:
Secreted.

Tissue Specificity:
T-cell and macrophage specific.

Post-translational modifications:
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.

Similarity:
Belongs to the intercrine beta (chemokine CC) family.

SWISS:
P13501

Gene ID:
6352

Database links:

Entrez Gene: 6352 Human

Omim: 187011 Human

SwissProt: P13501 Human

Unigene: 514821 Human



產(chǎn)品圖片
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCL5/RANTES Polyclonal Antibody, Unconjugated(bs-1324R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCL5/RANTES Polyclonal Antibody, Unconjugated(bs-1324R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RANTES) Polyclonal Antibody, Unconjugated (bs-1324R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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