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VEGF-C Rabbit pAb (bs-1586R)  
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產(chǎn)品編號 bs-1586R
英文名稱 VEGF-C Rabbit pAb
中文名稱 血管內(nèi)皮生長因子C型抗體
別    名 Vascuoar endothelial growth factor-C; AW228853; Flt4 ligand; Flt4-L; VEGF2; VEGFC; VRP; VEGFC_HUMAN.  
Specific References  (9)     |     bs-1586R has been referenced in 9 publications.
[IF=7.84] Zhuo, Wei, et al. "The CXCL12?CCXCR4 Chemokine Pathway: A Novel Axis Regulates Lymphangiogenesis."Clinical Cancer Research 18.19 (2012): 5387-5398.l  Human, Mouse.  
[IF=5.62] He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015).  WB ;  Human.  
[IF=4.302] Alunno et al. Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sj?gren's syndrome. (2016) J.Cell.Mol.Med. 20:613-22  IHC-P ;  Human.  
[IF=4.01] Alunno, Alessia, et al. "Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sj?grens syndrome." Journal of Cellular and Molecular Medicine (2016).  IHC-P ;  Human.  
[IF=3.575] Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 Dec;:  WB,IHC ;  Human,Mouse.  
[IF=2.82] Shin et al. Expression of vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) in the glial reaction elicited by human mesenchymal stem cell engraftment in the normal rat brain. (2015) J.Histochem.Cytochem. 63:170-80  IHC ;  Human.  
[IF=2.679] Michiko Jo. et al. Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping. MICROCIRCULATION. 2022 Oct;:e12788  WB ;  Rat.  
[IF=0.803] Ferr?o JSP et al. Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization. Vet Med Sci.?2019 Feb 11.  IHC-P ;  Mouse.  
[IF=0.72] Cialdai, Francesca, et al. "Modeled Microgravity Affects Fibroblast Functions Related to Wound Healing." Microgravity Science and Technology: 1-12.  WB ;  Mouse.  
研究領(lǐng)域 細(xì)胞生物  血管內(nèi)皮細(xì)胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 46 kDa
檢測分子量
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human VEGF-C: 321-415/415 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Vascular endothelial growth factors (VEGFs), also known as vasculotropins, are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. The target cell specificity of VEGF is restricted to vascular endothelial cells. Vascular Endothelial Growth Factor C (VEGFC) is a member of the VEGF subfamily of PDGF-related growth factors. It is the ligand for Flt4 (VEGFR3) and KDR (VEGFR2). VEGFC binds Flt4 and induces tyrosine autophosphorylation of VEGFR3 and VEGFR2. VEGFC also stimulates the migration of bovine capillary endothelial cells in collagen gel. It is a specific growth factor for the lymphatic vascular system and mediates lymphangiogenesis. VEGFC is abundantly expressed in heart and skeletal muscle. Other tissues such as lung and kidney also express VEGFC.

Subunit:
Homodimer; non-covalent and antiparallel.

Subcellular Location:
Secreted.

Tissue Specificity:
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P49767

Gene ID:
7424

Database links:

Entrez Gene: 7424 Human

Entrez Gene: 22341 Mouse

Entrez Gene: 114111 Rat

Omim: 601528 Human

SwissProt: P49767 Human

SwissProt: P97953 Mouse

SwissProt: O35757 Rat

Unigene: 435215 Human

Unigene: 1402 Mouse

Unigene: 6913 Rat



產(chǎn)品圖片
Sample: Lane 1: Lymph node (Mouse) Lysate at 40 ug Lane 2: Kidney (Mouse) Lysate at 40 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Lymph node (Rat) Lysate at 40 ug Lane 5: Kidney (Rat) Lysate at 40 ug Primary: Anti-VEGF-C (bs-1586R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (VEGF-C) Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-VEGF-C antibody (bs-1586R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-VEGF-C antibody (bs-1586R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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