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ox-LDL Rabbit pAb (bs-1698R)  
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產品編號 bs-1698R
英文名稱 ox-LDL Rabbit pAb
中文名稱 氧化低密度脂蛋白抗體
別    名 ox-LDL: LDL(Copper oxidized); Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein.  氧化型低密度脂蛋白;
Specific References  (11)     |     bs-1698R has been referenced in 11 publications.
[IF=31.373] Haikuo Li. et al. Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis. CELL METAB. 2022 Oct;:  IF ;  Human.  
[IF=18.699] Martinez, Jennifer, et al. "Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins." Nature Cell Biology (2015).  ICF ;  Mouse.  
[IF=7.396] Takuya Tsumita. et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. INT J CANCER. 2022 May 24  IF ;  Mouse.  
[IF=6.706] Qing Li. et al. Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets. NUTRIENTS. 2023 Jan;15(4):832  IHC ;  Mouse.  
[IF=4.473] Xiaosu Song. et al. Long noncoding RNA THRIL promotes foam cell formation and inflammation in macrophages. CELL BIOL INT. 2022 Oct;:  Other ;  Other.  
[IF=4.39] Wen, Song, et al. "OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice." Journal of Lipid Research 53.5 (2012): 829-838.  IHC-F ;  Mouse.  
[IF=4.164] Fen Gao. et al. Angiotensin II induces the expression of periostin to promote foam cell formation in oxLDL-treated macrophages. Int J Cardiol. 2022 Jan;347:46  ELISA ;  Human.  
[IF=3.876] Wang, Xin-Yuan. et al. Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study. Lipids Health Dis. 2021 Dec;20(1):1-13  IHC ;  Human.  
[IF=3.727] Song, Bangrong. et al. Differential Expression of LOXL1-AS1 in Coronary Heart Disease and its Regulatory Mechanism in ox-LDL-Induced Human Coronary Artery Endothelial Cell Pyroptosis. 2021 Oct 11  other ;  Human.  
[IF=3.661] Liu Chan-Jung. et al. Increased renal uptake and urine excretion of oxidized LDL is possibly associated with formation of large calcium oxalate nephrolithiasis: a preliminary study. WORLD J UROL. 2023 Mar;:1-8  IHC ;  Human.  
[IF=3.417] Xia Zhanget al. NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR?638/AKT/mTOR signaling in atherosclerosis. Oncol Rep . 2020 Jul;44(1):115-125.  other ;  
研究領域 心血管  細胞生物  免疫學  脂蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31 kDa
檢測分子量
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Full length protein from human plasma 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.
When too much LDL cholesterol circulates in the blood, it can slowly build up in the inner walls of the arteries that feed the heart and brain. Together with other substances it can form plaque, a thick, hard deposit that can clog those arteries. This condition is known as atherosclerosis. Oxidized lipoproteins are formed by free radical damage to lipids that accumulate in macrophages and smooth muscle cells causing foam cell formation, an initial step in the disease.

Subcellular Location:
Secreted (probable)

Gene ID:
ox-LDL

氧化低密度脂蛋白是低密度脂蛋白(low density lipoprotein,LDL)經氧化修飾后形成的,經研究認為:OXLDL的增高與動脈粥樣硬化性心血管病(Arteriosclerosis cardiovascular disease,ASCVD)的發生有密切關系。
產品圖片
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (bs-1698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:HepG2. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (bs-1698R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (bs-1698R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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