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ECE2 Rabbit pAb (bs-1753R)  
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產品編號 bs-1753R
英文名稱 ECE2 Rabbit pAb
中文名稱 內皮素轉化酶2抗體
別    名 ECE2; MGC78487; ECE 2; Endothelin converting enzyme 2; Hypothetical protein MGC2408; KIAA0604; MGC17664; ECE2_HUMAN.  
Specific References  (1)     |     bs-1753R has been referenced in 1 publications.
[IF=5.23] Esposito, Giuseppe, et al. "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration." Scientific reports 6 (2016).  IHC-F ;  Rat.  
研究領域 免疫學  神經生物學  轉錄調節因子  Alzheimer's  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 80-100 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ECE2: 601-700/883 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Endothelin-converting enzyme 2 (ECE2) converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity. Expressed at high levels in central nervous system. Expressed in adrenal glands, ovary and uterus, and at low levels in heart.

Subcellular Location:
Golgi apparatus membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic granule membrane; Single-pass type II membrane protein (By similarity).

Tissue Specificity:
Expressed in brain. Strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level).

Similarity:
In the N-terminal section; belongs to the methyltransferase superfamily.
In the C-terminal section; belongs to the peptidase M13 family.

SWISS:
P0DPD6

Gene ID:
9718

Database links:

Entrez Gene: 281134 Cow

Entrez Gene: 9718 Human

Omim: 610145 Human

SwissProt: Q10711 Cow

SwissProt: O60344 Human

Unigene: 146161 Human



產品圖片
Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-ECE2 (bs-1753R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80-100 kD Observed band size: 80 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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