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CXCL13 Rabbit pAb (bs-2553R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產品編號 bs-2553R
英文名稱 CXCL13 Rabbit pAb
中文名稱 B-淋巴細胞趨化因子抗體
別    名 ANGIE; ANGIE2; B cell attracting chemokine 1; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; BCA-1; BLC; BLR1L; C-X-C motif chemokine 13; Chemokine(C-X-C motif) ligand 13; Chemokine(C-X-C motif) ligand 13(B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; CXL13_HUMAN; SCYB13; Small inducible cytokine B subfamily(Cys-X-Cys motif), member 13(B-cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13; Small-inducible cytokine B13.  
Specific References  (8)     |     bs-2553R has been referenced in 8 publications.
[IF=8.98] Pei, Guangchang, et al. "Renal Interstitial Infiltration and Tertiary Lymphoid Organ Neogenesis in IgA Nephropathy." Clinical Journal of the American Society of Nephrology (2013): CJN-01150113.  IHC-P ;  Human.  
[IF=5.736] Maria Chiara Trolese. et al. CXCL13/CXCR5 signalling is pivotal to preserve motor neurons in amyotrophic lateral sclerosis. Ebiomedicine. 2020 Dec;62:103097  IHC ;  Human.  
[IF=4.21] Pimenta, Erica Maria, et al. "IRF5 is a novel regulator of CXCL13 expression in breast cancer that regulates CXCR5+ B-and T-cell trafficking to tumor-conditioned media." Immunology and Cell Biology (2014).  other ;  Human.  
[IF=3.99] Li, Congya, et al. "Up-regulation of chemokine CXCL13 in systemic candidiasis." Clinical Immunology (2017).  IHC-P ;  Mouse.  
[IF=3.641]   IHC ;  human.  
[IF=3.641] Cao G et al. An imbalance between blood CD4+CXCR5+Foxp3+ Tfr cells and CD4+CXCR5+Tfh cells may contribute to the immunopathogenesis of rheumatoid arthritis. Mol Immunol. 2020 Sep;125:1-8.  IHC-P ;  Human.  
[IF=3.641] Gan Caoet al. An imbalance between blood CD4+ CXCR5+ Foxp3+ Tfr cells and CD4+ CXCR5+ Tfh cells may contribute to the immunopathogenesis of rheumatoid arthritis. Mol Immunol . 2020 Sep;125:1-8.  ELISA ;  Human.  
[IF=2.07] Lemma, Siria A., et al. "Similar chemokine receptor profiles in lymphomas with central nervous system involvement–possible biomarkers for patient selection for central nervous system prophylaxis, a retrospective study." European Journal of Haematology (2015).  IHC-P ;  Human.  
研究領域 細胞生物  免疫學  趨化因子  b-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 10 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CXCL13: 23-109/109 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.

Function:
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

Subcellular Location:
Secreted.

Tissue Specificity:
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

Similarity:
Belongs to the intercrine alpha (chemokine CxC) family.

SWISS:
O43927

Gene ID:
10563

Database links:

Entrez Gene: 10563 Human

Entrez Gene: 55985 Mouse

Entrez Gene: 498335 Rat

Omim: 605149 Human

SwissProt: O43927 Human

SwissProt: O55038 Mouse

Unigene: 100431 Human

Unigene: 10116 Mouse

Unigene: 6917 Rat



產品圖片
Sample: Lane 1: Human Jurkat cell lysates Primary: Anti-CXCL13 (bs-2553R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 10 kDa Observed band size: 16 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCL13) Polyclonal Antibody, Unconjugated (bs-2553R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Image was kindly submitted by Erica Pimenta from UMDNJ. Human breast tumor tissue stained with Rabbit Anti-CXCL13 Polyclonal Antibody (bs-2553R)
Blank control(black line):HepG2. Primary Antibody (green line): Rabbit Anti-CXCL13 antibody (bs-2553R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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