麻豆av资源_日本三级一区_www.狠狠艹_国产精妇在线观看第一区_成人性生交大片免费看中国A片_日本一本久久

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質量反饋  關于我們  聯系我們
亚洲双插,亚洲黄色网页,91亚洲国产在人线播放午夜
首頁 > 產品中心 > 一抗 > 產品信息
CENPA Rabbit pAb (bs-2753R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
50ul/1180.00元
100ul/1980.00元
大包裝/詢價

產品編號 bs-2753R
英文名稱 CENPA Rabbit pAb
中文名稱 著絲粒蛋白A
別    名 CENP A; CENP-A; Centromere autoantigen A; Centromere protein A 17kDa; centromere protein A; centromeric protein A; Histone H3 like centromeric protein A; cenpa; CENPA_HUMAN; Histone H3-like centromeric protein A.  
研究領域 細胞生物  染色質和核信號  細胞周期蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 15 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CENPA: 65-140/140 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].

Function:
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).

Subunit:
Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts (via CATD domain) with HJURP; the interaction is direct and is required for its localization to centromeres. Interacts with CENPC, CENPN and CENPT; interaction is direct. Interacts directly with herpes virus HSV-1 ICP0 protein. Part of a centromere complex consisting of CENPA, CENPT and CENPW.

Subcellular Location:
Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.

Tissue Specificity:
Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by AURKA and AURKB during prophase is required for localization of AURKA and AURKB at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by AURKA and is maintained by AURKB.
Poly-ADP-ribosylated by PARP1.

Similarity:
Belongs to the histone H3 family.

SWISS:
P49450

Gene ID:
1058

Database links:

Entrez Gene: 1058 Human

Omim: 117139 Human

SwissProt: P49450 Human

Unigene: 1594 Human



CENP-A可將著絲粒變成一個DNA和蛋白的復合物,而且確保著絲粒在細胞分裂中完好無損。
CENP-A的存在確保了人體有幾乎完全相同的染色體組。
CENP-A功能失常將導致細胞分裂過程中發生染色體分離異常。
CENP-A對于染色體著絲粒的位置起決定作用,因而被認為是重要的表觀遺傳學標記蛋白。
CENP-A改變了核小體的性狀,使得它變得更加堅硬。核小體是由DNA與組合蛋白八聚體構成的真核染色體的一種重復結構,核小體的組織對于基因調控極為重要。不同于染色質上的其他區域。
CENP-A取代組蛋白H3形成核小體,CENP-A核小體重復拷貝形成一個特異的表觀遺傳區域。
產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPA) Polyclonal Antibody, Unconjugated (bs-2753R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(black line):NIH/3T3. Primary Antibody (green line): Rabbit Anti-CENPA antibody (bs-2753R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版權所有 2004-2026 m.jywcc.com 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號
主站蜘蛛池模板: 成年视频女人的天堂天天看片 | 欧美精品久 | 亚洲综合另类小说色区一 | 日日噜噜夜夜狠狠久久无码区 | 国产福利91精品一区二区 | 中文字幕久久久 | 日韩大片在线永久观看视频网站免费 | 国产特黄大片aaaaa毛片 | 精品国产区一区二区三区在线观看 | hhh在线观看 | a片久久久久久久久久久久 免费观看日韩精品 | 亚洲三区视频在线观看 | 激情在线播放 | 亚州精品国产 | 亚日韩精品| 欧美人妻少妇精品久久黑人 | 亚洲国产一区二区精品专区 | 91精品一区二区在线观看 | 兔子先生第三季免费视频播放 | 男女啪啪抽搐一进区 | 青青成线在人线免费啪 | 蜜桃传媒视频麻豆第一区免费观看 | 日韩在线观看成人 | 欧美人与性动交CCOO | 亚洲美女高潮久久久久 | 婷婷天堂网 | 不卡视频在线 | 成人18视频日本 | 超碰97色 | 国产高潮成人免费视频在线观看 | 欧美一区二区三区激情 | 最新中文字幕一区二区三区 | 在线观看成人高清a | 久久久久久噜噜噜久久久精品 | 成人精品av一区二区三区网站 | 国产精品亚洲a∨天堂 | 日本精品一区二区三区香蕉久久 | 国产91免费观看 | 亚洲婷婷综合色高清在线 | 亚洲精品成AV人片天堂无码 | 亚洲国产高清在线播放 |