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Phospho-TH (Ser31) Rabbit pAb (bs-3463R)  
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產(chǎn)品編號 bs-3463R
英文名稱 Phospho-TH (Ser31) Rabbit pAb
中文名稱 磷酸化酪氨酸羥化酶抗體
別    名 Tyrosine Hydroxylase(phospho-Ser31); Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_RAT; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase.  
產(chǎn)品類型 磷酸化抗體 
研究領域 腫瘤  細胞生物  免疫學  神經(jīng)生物學  信號轉(zhuǎn)導  內(nèi)分泌病  細胞類型標志物  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 56 kDa
檢測分子量
細胞定位 細胞核 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from rat TH around the phosphorylation site of Ser31: VT(p-S)PR 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.

Function:
Plays an important role in the physiology of adrenergic neurons.

Subunit:
Homotetramer.

Subcellular Location:
Mainly expressed in the brain and adrenal glands.

Post-translational modifications:
In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.

Similarity:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

SWISS:
P04177

Gene ID:
25085

Database links:

Entrez Gene: 7054 Human

Entrez Gene: 21823 Mouse

Entrez Gene: 25085 Rat

Omim: 191290 Human

SwissProt: P07101 Human

SwissProt: P24529 Mouse

SwissProt: P04177 Rat

Unigene: 435609 Human

Unigene: 1292 Mouse

Unigene: 11082 Rat



神經(jīng)細胞標志物
酪氨酸羥化酶(TH)是兒茶酚胺類神經(jīng)遞質(zhì)即多巴胺、去甲腎上腺素、腎上腺素生物合成過程所需的限速酶,它以四氫生物喋呤啶(BH4)為輔酶,催化酪氨酸的羥化而生成多巴(DOPA)。
已知在患帕金森病(Parkinson disease,PD)時,腦內(nèi)多巴胺(dopamine,DA)的減少與此酶活性低下有關。因此對PD模型動物來說,若將TH基因植入腦內(nèi),便可以提高腦內(nèi)DA水平而達到基因治療目的。
產(chǎn)品圖片
Sample: Lane 1: Adrenal gland (Mouse) Lysate at 40 ug Lane 2: Adrenal gland (Rat) Lysate at 40 ug Primary: Anti-Phospho-TH (Ser31) (bs-3463R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (bs-3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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