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phospho-HNF4 (Ser313) Rabbit pAb (bs-4001R)  
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產品編號 bs-4001R
英文名稱 phospho-HNF4 (Ser313) Rabbit pAb
中文名稱 磷酸化肝細胞核因子4α抗體
別    名 HNF4(phospho S313); p-HNF4(phospho S313); Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; HNF 4 alpha; HNF 4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF 14; TCF; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF4.  
Specific References  (2)     |     bs-4001R has been referenced in 2 publications.
[IF=3.776] Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs. HELIYON. 2022 Dec;8:e12385  WB ;  Human.  
[IF=2.523] Zhang C et al. Caveolin-1 promotes Rfng expression via Erk-Jnk-p38 signaling pathway in mouse hepatocarcinoma cells. J Physiol Biochem. 2019 Sep 16.  WB ;  Mouse.  
產品類型 磷酸化抗體 乙酰化抗體 
研究領域 腫瘤  免疫學  染色質和核信號  信號轉導  轉錄調節因子  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,GuineaPig,Horse)
產品應用 WB=1:500-2000,Flow-Cyt=2ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 52 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human HNF4 alpha around the phosphorylation site of Ser313: LR(p-S)QV 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.

Function:
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Subunit:
Homodimerization is required for HNF4-alpha to bind to its recognition site.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.
Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

DISEASE:
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

Similarity:
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P41235

Gene ID:
3172

Database links:

Entrez Gene: 3172 Human

Entrez Gene: 15378 Mouse

Entrez Gene: 25735 Rat

Omim: 600281 Human

SwissProt: P41235 Human

SwissProt: P49698 Mouse

SwissProt: P22449 Rat

Unigene: 116462 Human

Unigene: 202383 Mouse

Unigene: 12238 Rat



產品圖片
Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Rat Stomach tissue lysates Primary: Anti-phospho-HNF4 (Ser313) (bs-4001R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 50 kDa
Blank control(black line):HUVEC. Primary Antibody (green line): Rabbit Anti-phospho-HNF4 (Ser313) antibody (bs-4001R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):HepG2. Primary Antibody (green line): Rabbit Anti-phospho-HNF4 (Ser313) antibody (bs-4001R) Dilution:2ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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