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CYP24A1 Rabbit pAb (bs-3899R)  
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產(chǎn)品編號(hào) bs-3899R
英文名稱 CYP24A1 Rabbit pAb
中文名稱 細(xì)胞色素P450 24A1抗體
別    名 1 25 dihydroxyvitamin D3 24 hydroxylase; 1 25 dihydroxyvitamin D(3) 24 hydroxylase; 1; 1, 25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24 ohase; 24-OHase; 25-dihydroxyvitamin D(3) 24-hydroxylase; CP 24; CP24; CP24A_HUMAN; CYP 24; CYP24; CYP24A1; Cytochrome P450 24A1; Cytochrome P450 24A1 mitochondrial; Cytochrome P450 family 24; Cytochrome P450 family 24 subfamily A polypeptide 1; Cytochrome P450 subfamily XXIV; Cytochrome P450, subfamily XXIV(vitamin D 24-hydroxylase); Cytochrome P450-CC24; EC 1.14.13.n4; Exo mitochondrial protein; HCAI; MGC126273; MGC126274; mitochondrial; P450 CC24; Vitamin D 24 hydroxylase; Vitamin D(3) 24 hydroxylase; Vitamin D(3) 24-hydroxylase.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  生長因子和激素  內(nèi)分泌病  脂蛋白  新陳代謝  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CYP24A1: 351-450/514 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1) is a member of the cytochrome P450 superfamily. It is a mitochondrial enzyme responsible for inactivating vitamin D3 metabolites through the C-24 oxidation pathway. In regulating the level of vitamin D3, CYP24A1 plays a role in calcium homeostasis and the vitamin D endocrine system. CYP24A1 also has a role in maintaining calcium homeostasis. It catalyzes the NADPH-dependent 24-hydroxylation of 25-hydroxyvitamin D(3) in the presence of adrenodoxin and NADPH-adrenodoxin reductase.

Function:
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.

Subcellular Location:
Mitochondrion.

DISEASE:
Hypercalcemia infantile (HCAI) [MIM:143880]: A disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
Q07973

Gene ID:
1591

Database links:

Entrez Gene: 1591 Human

Entrez Gene: 13081 Mouse

Omim: 126065 Human

SwissProt: Q07973 Human

SwissProt: Q64441 Mouse

Unigene: 89663 Human

Unigene: 6575 Mouse



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CYP24A1) Polyclonal Antibody, Unconjugated (bs-3899R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CYP24A1) Polyclonal Antibody, Unconjugated (bs-3899R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CYP24A1) Polyclonal Antibody, Unconjugated (bs-3899R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP24A1) polyclonal Antibody, Unconjugated (bs-3899R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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