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PYGM Rabbit pAb (bs-5012R)  
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產品編號 bs-5012R
英文名稱 PYGM Rabbit pAb
中文名稱 肌肉糖原磷酸化酶抗體
別    名 Glycogen phosphorylase muscle form; Muscpho; Myophosphorylase; phosphorylase glycogen muscle(McArdle syndrome glycogen storage disease type V); PYGM_HUMAN.  
Specific References  (1)     |     bs-5012R has been referenced in 1 publications.
[IF=9.231] Yuqiang Bai. et al. Phosphorylation and acetylation responses of glycolytic enzymes in meat to different chilling rates. FOOD CHEM. 2023 Mar;:135896  WB ;  Sheep.  
研究領域 腫瘤  細胞生物  免疫學  轉錄調節因子  糖蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 97 kDa
檢測分子量
細胞定位 細胞漿 細胞外基質 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PYGM: 401-500/842 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Function:
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Subunit:
Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.

Post-translational modifications:
Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

DISEASE:
Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.

Similarity:
Belongs to the glycogen phosphorylase family.

SWISS:
P11217

Gene ID:
5837

Database links:

Entrez Gene: 5837 Human

Entrez Gene: 19309 Mouse

Entrez Gene: 24701 Rat

Omim: 608455 Human

SwissProt: P11217 Human

SwissProt: Q9WUB3 Mouse

SwissProt: P09812 Rat

Unigene: 154084 Human

Unigene: 27806 Mouse

Unigene: 11238 Rat



產品圖片
Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Rat Muscle tissue lysates Primary: Anti-PYGM (bs-5012R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kD Observed band size: 97 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (bs-5012R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (bs-5012R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (bs-5012R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (bs-5012R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (bs-5012R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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