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CREM Rabbit pAb (bs-5135R)  
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產品編號 bs-5135R
英文名稱 CREM Rabbit pAb
中文名稱 環磷酸腺苷反應元件調節蛋白抗體
別    名 cAMP response element modulator; CREM-1; cAMP responsive element modulator; hCREM 2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893.  
研究領域 腫瘤  免疫學  轉錄調節因子  激酶和磷酸酶  結合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Pig,Chicken,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CREM: 251-361/361 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 CREM is a a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is derived from a multiexonic gene that encodes both activators and antagonists of cAMP-inducible transcription by differential splicing. Splice variants with antagonistic function lack 2 glutamine-rich domains and block cAMP-induced transcription, whereas an isoform that includes these glutamine-rich domains is a transcriptional activator.

Function:
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation (By similarity).

Subunit:
Binds DNA as a dimer. Interacts with FHL5. Interacts with CDC34.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.

Post-translational modifications:
Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
Stimulated by phosphorylation.

Similarity:
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.

SWISS:
Q03060

Gene ID:
1390

Database links:

Entrez Gene: 1390 Human

Entrez Gene: 12916 Mouse

Entrez Gene: 25620 Rat

Omim: 123812 Human

SwissProt: Q03060 Human

SwissProt: P27699 Mouse

SwissProt: Q03061 Rat

Unigene: 200250 Human

Unigene: 5244 Mouse

Unigene: 10251 Rat



CREM是一種與CREB高度同源的蛋白,與CREB均一表達于幾種細胞類型不同,其表達具有細胞特異性,CREM同CREB一樣能高效特異性結合到CRE元件上,但相反的是其下調cAMP誘導的轉錄.
產品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CREM) Polyclonal Antibody, Unconjugated (bs-5135R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CREM) Polyclonal Antibody, Unconjugated (bs-5135R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CREM) Polyclonal Antibody, Unconjugated (bs-5135R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREM) Polyclonal Antibody, Unconjugated (bs-5135R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CREM) polyclonal Antibody, Unconjugated (bs-5135R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:A549. Primary Antibody (green line): Rabbit Anti-CREM antibody (bs-5135R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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