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DAB kit (20×) (C-0010)  
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0.5ml×2/70.00元
1ml×2/120.00元
5ml×2/280.00元
大包裝/詢價

產品編號 C-0010
英文名稱 DAB kit (20×)
中文名稱 DAB顯色試劑盒(20×)
別    名 DAB Substrate; DAB Horseradish Peroxidase Color Development Kit; DAB HRP Color Development Kit;   DAB辣根過氧化物酶顯色試劑盒; DAB顯色試劑盒; 辣根過氧化物酶(HRP)底物; C02-04001; C24005; DAB試劑盒;
Specific References  (71)     |     C-0010 has been referenced in 71 publications.
[IF=21.95] Shi L et al. A Self‐Pumping Dressing for Draining Excessive Biofluid around Wounds. Adv Mater. 2019 Feb;31(5):e1804187.  IHC-P ;  
[IF=10.75] Yao Xinlei. et al. Tectorigenin targets PKACα to promote GLUT4 expression in skeletal muscle and improve insulin resistance in vitro and in vivo. INT J BIOL SCI. 2023 Mar;19(5):1579-1596  
[IF=8.355] Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109.  IHC ;  
[IF=8.355] Xie S et al. Bacteria-propelled microrockets to promote the tumor accumulation and intracellular drug uptake. Chemical Engineering Journal,2019, 123786.  IHC ;  
[IF=7.634] Ma Yubo. et al. Immature rat testis sustained long-term development using an integrative model. BIOL RES. 2022 Dec;55(1):1-13  Other ;  Other.  
[IF=7.419] Dai Cao. et al. Artesunate promoted anti-tumor immunity and overcame EGFR-TKI resistance in non-small-cell lung cancer by enhancing oncogenic TAZ degradation. BIOMED PHARMACOTHER. 2022 Nov;155:113705  Other ;  Other.  
[IF=6.543] Ma Yu-Bo. et al. Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation. Oxid Med Cell Longev. 2021;2021:1699990  Other ;  Rat.  
[IF=6.491] Song J et al. Deleting MyD88 signaling in myeloid cells promotes development of adenocarcinomas of the colon.Cancer Lett. 2018 Oct 1;433:65-75.  IHC ;  
[IF=6.383] Xie S et al. Bacterial microbots for acid-labile release of hybrid micelles to promote the synergistic antitumor efficacy.Acta Biomater. 2018 Sep 15;78:198-210.  IHC-P ;  
[IF=6.304] Nan Wang. et al. PRMT5/Wnt4 axis promotes lymph-node metastasis and proliferation of laryngeal carcinoma. Cell Death Dis. 2020 Oct;11(10):1-15  
[IF=6.208] Yining Zhang. et al. Activating Wnt/β-Catenin Signaling in Osteocytes Promotes Osteogenic Differentiation of BMSCs through BMP-7. INT J MOL SCI. 2022 Jan;23(24):16045  
[IF=6.208] Baohui Yao. et al. Reproductive Suppression Caused by Spermatogenic Arrest: Transcriptomic Evidence from a Non-Social Animal. INT J MOL SCI. 2023 Jan;24(5):4611  
[IF=5.793] Yanan Shen. et al. Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice. J Neuroinflamm. 2020 Dec;17(1):1-16  IHC ;  Mouse.  
[IF=5.506] Wang Shufen. et al. FOXD3 confers chemo-sensitivity in ovarian cancer through a miR-335/DAAM1/myosin II axis-dependent mechanism. J OVARIAN RES. 2023 Dec;16(1):1-15  
[IF=5.5] Zhang Y et al. MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway.Cell Physiol Biochem. 2018;48(1):348-360.  IHC-P ;  
[IF=5.5] Yu JL et al. Lentivirus-Mediated Gene Silencing of Lymphocyte Function-Associated Antigen 1 Inhibits Apoptosis of Hippocampal Neurons in Rats with Acute Cerebral Ischemia After Cerebral Lymphatic Blockage.(2018) Cell Physiol Biochem.51(3):1069-1086.  IHC-P ;  
[IF=5.391] Yang C et al. Long?noncoding?RNA?HAGLR?acts?as a?microRNA-143-5p?sponge?to?regulate?epithelial-mesenchymal?transition?and?metastatic?potential?in?esophageal?cancer?by regulating LAMP3. FASEB J. 2019 Jul 15:fj201802543RR.  IHC-P ;  
[IF=5.076] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498.  IHC ;  
[IF=5.047] Chen Z et al. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release. Journal of Materials Chemistry B.2019.  IHC-P ;  
[IF=4.831] Ying Xiao. et al. LINC00265 targets miR-382-5p to regulate SAT1, VAV3 and angiogenesis in osteosarcoma.. Aging-Us. 2020 Oct 31; 12(20): 20212–20225  IHC ;  Human.  
[IF=4.694] Qi SS et al. Protective Effects of Chromium Picolinate Against Diabetic-Induced Renal Dysfunction and Renal Fibrosis in Streptozotocin-Induced Diabetic Rats. Biomolecules. 2020 Mar 4;10(3).  staining ;  rat.  
[IF=4.658] Wang AH et al. Down‐regulation of long non‐coding RNA HOTAIR inhibits invasion and migration of oesophageal cancer cells via up‐regulation of microRNA‐204. J Cell Mol Med. 2019 Aug 7.  IHC ;  
[IF=4.545] Dai Caoet al. Luteolin suppresses epithelial-mesenchymal transition and migration of triple-negative breast cancer cells by inhibiting YAP/TAZ activity. Biomed Pharmacother . 2020 Sep;129:110462.  other ;  
[IF=4.522] Ma S et al. Histone?deacetylases?inhibitor?MS-275?suppresses?human?esophageal?squamous?cell?carcinomacell?growth?and?progression?via?the?PI3K/Akt/mTOR?pathway. J Cell Physiol. 2019 May 23.  IHC-P ;  
[IF=4.486] Wang N et al. Dysregulated YY1/PRMT5 axis promotes the progression and metastasis of laryngeal cancer by targeting Hippo pathway  IHC ;  
[IF=4.432] Pengfei Cheng. et al. A3 adenosine receptor agonist IB-MECA reverses chronic cerebral ischemia-induced inhibitory avoidance memory deficit. Eur J Pharmacol. 2022 Apr;921:174874  Other ;  Other.  
[IF=4.255] Chen LD et al. T-cell death-associated gene 8 accelerates atherosclerosis by promoting vascular smooth muscle cell proliferation and migration. Atherosclerosis. 2020 Jan 22;297:64-73.  IHC ;  
[IF=3.998] Hongbin Guo. et al. Metabolites profiling and pharmacokinetics of troxipide and its pharmacodynamics in rats with gastric ulcer. Sci Rep-Uk. 2020 Aug;10(1):1-21  other ;  
[IF=3.973] Luo X et al. Encapsulation of Escherichia coli strain Nissle 1917 in a chitosan―alginate matrix by combining layer-by-layer assembly with CaCl2 cross-linking for an effective treatment of inflammatory bowel diseases. Colloids Surf B Biointerfaces.?2020 Jan 21;189:110818.  IHC-P ;  
[IF=3.923] Liu?L et al. CP-31398 inhibits the progression of cervical cancer through reversing the epithelial mesenchymal transition via the downregulation of PAX2s. J Cell Physiol. 2019 Mar;234(3):2929-2942.  IHC ;  
克 隆 號
CAS
理論分子量 kDa
檢測分子量
保存條件 Store at –20℃.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 DAB (3,3′-Diaminobenzidine; 3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl) is Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually. The kit contains three components: the DAB Chromogen, Substrate Buffer and Staining Assistant. All components are in liquid form. This new 3-Solution DAB product has improved stability, ease of formulation, and greater sensitivity than previous DAB products.

DAB,即3,3’-二氨基聯苯胺(3,3’-diaminobenzidine)是辣根過氧化物酶(Peroxidase)的常用生色底物,在過氧化氫的存在下失去電子而呈現出顏色變化,形成淺棕色不溶性產物。用于檢測過氧化物酶的活性,它靈敏度高,特異性好,是HRP結合物最常用的底物,常在免疫組化,原位雜交,Western?blot等膜顯色或檢測細胞或組織內源性的過氧化物酶中應用廣泛。
用法:
1. 臨用前配制 10 mL 1×DAB 顯色工作液:將 0.5 mL 20× DAB 顯色液 A 加入 9 mL 1×PBS 中并渦旋混勻。向 DAB / PBS 混合物中加入0.5 mL 20×DAB顯色液B渦旋混合均勻。 可以根據需要按比例縮放體積。 注意:不要使用含有疊氮化鈉的緩沖液,疊氮化鈉是一種HRP抑制劑。
2. 在樣品的最后一次洗滌完畢后,去除洗滌液,加入足夠 的 DAB 顯色工作液,確保能充分覆蓋樣品。室溫避光孵育 3-30 分鐘或更長時間,直至顯色達到預期深淺。免疫印跡實驗,應保證樣品在一個足夠多的染色液中且能被自由晃動。
3. 去除 DAB 染色工作液,用 PBS 沖洗樣品幾次以中止顯色反應。對于用顯微鏡觀察的細胞或組織樣品,可復染樣品(可選)并安裝在水性封固介質中。或者,可以將樣品脫水并安裝在有機封固劑中。免疫印跡實驗,可將樣品用水沖洗,風干,然后在室溫下儲存。

儲存與運輸:
-20℃避光密閉保存,一年有效。避免反復凍融。
2~8℃運輸,在途時間5天對試劑無影響。

注意事項
1. DAB對人體有害,操作時請小心,并注意有效防護以避免直接接觸人體或吸入體內。
2. 顯色工作液應現用現配,新鮮配制的工作液應為無色或淺棕色,如顏色過深,請勿使用。??
3. 顯色時間嚴格控制,根據情況調整,以免顯色過度。固相膜顯色數小時后即會褪色,不能永久存在。
4. 請將沾有DAB顯色液A的容器等放在含有3% KMnO4,2% NaHCO3?的溶液浸泡3h,以減少污染。?
5. 本產品僅限于專業人員的科學研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內。
6. 為了您的安全和健康,請穿實驗服并戴一次性手套操作。
產品圖片
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