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CD40/TNFRSF5 Rabbit pAb (bs-2929R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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產品編號 bs-2929R
英文名稱 CD40/TNFRSF5 Rabbit pAb
中文名稱 腫瘤壞死因子受體超家族成員5抗體
別    名 Tumor necrosis factor receptor superfamily, member 5; TNFRSF5; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40 antigen(TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; IMD3; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; p50 antibody T-BAM; TBAM; TNF receptor superfamily member 5; TNR5_HUMAN; TRAP antibody Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5 precursor; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a; AI326936.  
Specific References  (4)     |     bs-2929R has been referenced in 4 publications.
[IF=7.182] Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211  IHC ;  Mouse.  
[IF=6.819] Xinxin Yu. et al. Nanotherapy for bone repair: milk-derived small extracellular vesicles delivery of icariin. DRUG DELIV. 2023;30(1):Article: 2169414  WB ;  Cow.  
[IF=3.644] Dong Ming. et al. FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation. Front Bioeng Biotech. 2021 Feb;9:146  WB ;  Mouse.  
[IF=2.245] Dahan Yang. et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus. Front Vet Sci. 2021; 8: 666512  WB ;  Chicken.  
研究領域 心血管  細胞生物  免疫學  干細胞  細胞表面分子  淋巴細胞  b-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat,Rabbit (predicted: Pig,Cow,Chicken,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 30 kDa
檢測分子量 44-50
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD40/TNFRSF5.: 201-277/277 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Subcellular Location:
Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted.

Tissue Specificity:
B-cells and in primary carcinomas.

Similarity:
Contains 4 TNFR-Cys repeats.

SWISS:
P25942

Gene ID:
958

Database links:

Entrez Gene: 958 Human

Entrez Gene: 21939 Mouse

Entrez Gene: 171369 Rat

Omim: 109535 Human

SwissProt: P25942 Human

SwissProt: P27512 Mouse

Unigene: 472860 Human

Unigene: 271833 Mouse



產品圖片
Sample: Jurkat(Human) Cell Lysate at 30 ug Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-CD40(bs-2929R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 52 kD
Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-CD40(bs-2929R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 52 kD
Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Lymph node (Rat) Lysate at 40 ug Primary: Anti-CD40/TNFRSF5 (bs-2929R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44-50 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (bs-2929R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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