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RAB9 Rabbit pAb (bs-6177R)  
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產品編號 bs-6177R
英文名稱 RAB9 Rabbit pAb
中文名稱 ras癌基因家族Rab9蛋白抗體
別    名 DmRab9; Rab 9; RAB 9A; RAB9 member RAS oncogene family; RAB9A; RAB9A member RAS oncogene family; RAS ASSOCIATED PROTEIN RAB9; Ras related protein Rab 9A; Sid6061p; Sid99; RAB9A_HUMAN.  
研究領域 腫瘤  細胞生物  免疫學  信號轉導  G蛋白偶聯受體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Chicken,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RAB9: 65-170/201 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Endoplasmic reticulum membrane (Potential). Golgi apparatus membrane (Potential). Late endosome.

Similarity:
Belongs to the small GTPase superfamily. Rab family.

SWISS:
P51151

Gene ID:
9367

Database links:

Entrez Gene: 511776 Cow

Entrez Gene: 403947 Dog

Entrez Gene: 9367 Human

Entrez Gene: 56382 Mouse

Entrez Gene: 84589 Rat

Omim: 300284 Human

SwissProt: P24408 Dog

SwissProt: P51151 Human

SwissProt: Q9R0M6 Mouse

SwissProt: Q99P75 Rat

Unigene: 495704 Human

Unigene: 728400 Human

Unigene: 25306 Mouse

Unigene: 35289 Rat



產品圖片
Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-RAB9 (bs-6177R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD
Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-RAB9 (bs-6177R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD
Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-RAB9 (bs-6177R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RAB9 Polyclonal Antibody, Unconjugated(bs-6177R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB9) Polyclonal Antibody, Unconjugated (bs-6177R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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