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GBP5 Rabbit pAb (bs-7019R)  
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產品編號 bs-7019R
英文名稱 GBP5 Rabbit pAb
中文名稱 G蛋白結合蛋白5抗體
別    名 GBP 5; GBP-5; GBP TA antigen; GBP5; GBP-TA antigen; GBP5; GBP5_HUMAN; GTP binding protein 5; GTP-binding protein 5; Guanine nucleotide binding protein 5; Guanine nucleotide-binding protein 5; Guanylate binding protein 5; Guanylate-binding protein 5.  
研究領域 腫瘤  細胞生物  信號轉導  轉錄調節因子  G蛋白信號  干擾素  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 66 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GBP5/Guanylate binding protein 5: 221-320/586 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 GBP5 is a 586 amino acid protein that localizes to the cytoplasmic side of the cell membrane. Belonging to the interferon (IFN)-inducible guanylate-binding protein (GBP) family, GBP5 may be involved in the inflammatory response or with cell proliferation and is suggested to have possible cancer-related functions. The gene encoding GBP5 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma.

Function:
Stimulates the NLRP3 inflammasome assembly upon bacterial challenge.

Subunit:
Homotetramer when interacting with NLRP3 (By similarity). Homodimerizes upon GTP-binding, dimerization is required for the second hydrolysis step from GDP to GMP. Can also heterodimerize with other members of the family. Homotetramer when interacting with NLRP3.

Subcellular Location:
Cytoplasm. Membrane; Lipid-anchor.

Similarity:
Belongs to the GBP family.

SWISS:
Q96PP8

Gene ID:
115362

Database links:

Entrez Gene: 115362 Human

Entrez Gene: 229898 Mouse

Entrez Gene: 362050 Rat

Omim: 611467 Human

SwissProt: Q96PP8 Human

SwissProt: Q8CFB4 Mouse

Unigene: 513726 Human

Unigene: 459245 Mouse

Unigene: 224982 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GBP5) Polyclonal Antibody, Unconjugated (bs-7019R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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