| 產品編號 | bs-9431R |
| 英文名稱 | KCTD5 Rabbit pAb |
| 中文名稱 | 鉀離子通道四聚體結構域蛋白5抗體 |
| 別 名 | BTB/POZ domain-containing protein KCTD5; FLJ20040; KCTD5; KCTD5_HUMAN; Potassium channel tetramerisation domain containing 5. |
| 研究領域 | 細胞生物 免疫學 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KCTD5: 101-200/234 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. KCTD5 (potassium channel tetramerisation domain containing 5) is a 234 amino acid protein that localizes predominantly in the cytoplasm but translocates to the nucleus upon interaction with REP proteins. Existing as a homopentamer and consisting of one BTB (POZ) domain, KCTD5 associates with GRASP55, CUL-3 and ubiquitinated proteins. Interaction with CUL-3 suggests KCTD5 functions as a substrate adapter protein in some E3 ligase complexes.
Subunit: Homopentamer. Interacts (via C-terminus) with GRASP55/GORASP2. Interacts with CUL3 and with ubiquitinated proteins. Interacts with adeno-associated virus 2 (AAV-2) REP proteins. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Predominantly cytoplasmic, translocated to the nucleus upon interaction with Rep proteins. Similarity: Contains 1 BTB (POZ) domain. SWISS: Q9NXV2 Gene ID: 54442 Database links: Entrez Gene: 54442 Human Omim: 611285 Human SwissProt: Q9NXV2 Human Unigene: 61960 Human |
| 產品圖片 |
Tissue/cell: Rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KCTD5 Polyclonal Antibody, Unconjugated(bs-9431R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
