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Metallothionein 3 Rabbit pAb (bs-4940R)  
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產(chǎn)品編號 bs-4940R
英文名稱 Metallothionein 3 Rabbit pAb
中文名稱 金屬硫蛋白3抗體
別    名 Mt3; Mt-3; Mt 3; GIFB; GRIF; Metallothionein III; GIF; Growth inhibitory factor; Metallothionein3; Metallothionein-3; Metallothionein-III; MT-III; MT III; MT3_HUMAN.  
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  神經(jīng)生物學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  鋅指蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 7.5 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Metallothionein 3: 1-68/68 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 MT3, Metallothionein 3, binds heavy metals. It contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. MT3 inhibits survival and neurite formation of cortical neurons in vitro.

SWISS:
P25713

Gene ID:
4504

Database links:

Entrez Gene: 4504 Human

Entrez Gene: 17751 Mouse

Entrez Gene: 117038 Rat

Omim: 139255 Human

SwissProt: P25713 Human

SwissProt: P28184 Mouse

SwissProt: P37361 Rat

Unigene: 73133 Human

Unigene: 2064 Mouse

Unigene: 11325 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Metallothionein 3) Polyclonal Antibody, Unconjugated (bs-4940R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Metallothionein 3) Polyclonal Antibody, Unconjugated (bs-4940R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control:A549. Primary Antibody (green line): Rabbit Anti-Metallothionein 3 antibody (bs-4940R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution:0.2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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