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phospho-ZCWCC1 (Ser739) Rabbit pAb (bs-10016R)  
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產品編號 bs-10016R
英文名稱 phospho-ZCWCC1 (Ser739) Rabbit pAb
中文名稱 磷酸化ZCWCC1抗體
別    名 AC004542.C22.1.; p-MORC2(Ser739); phospho-ZCWCC1(Ser739); CW type with coiled coil domain 1; KIAA0852; ZCW3; ZCWCC1; Zinc finger; Zinc finger CW type coiled coil domain protein 1; Zinc finger CW type with coiled coil domain 1; Zing finger CW type 3 zinc finger CW-type coiled-coil domain protein 1; MORC family CW-type zine finger 2; MORC2.  
Specific References  (1)     |     bs-10016R has been referenced in 1 publications.
[IF=5.17] Wang et al. PAK1-mediated MORC2 phosphorylation promotes gastric tumorigenesis. (2015) Oncotarge. 6:9877-86  WB, IHC-P ;  Human, Mouse.  
產品類型 磷酸化抗體 
研究領域 細胞生物  轉錄調節因子  鋅指蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat (predicted: Mouse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 114 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human MORC2 around the phosphorylation site of Ser739: KR(p-S)VA 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The CW domain is a structural module found in many vertebrate, parasitic and plant proteins. It consists of a mononuclear four-cysteine zinc-finger domain that plays a role in DNA binding, chromatin methylation and early embryonic development. ZCWCC1 (zinc finger CW-type coiled-coil domain protein 1), also known as MORC2 (MORC family CW-type zinc finger protein 2) or ZCW3, is a 1,032 amino acid protein that contains one CW-type zinc finger domain. ZCWCC1 is located on chromosome 22 and is ubiquitously expressed with highest expression in pancreas, smooth muscle and testis. Expression of ZCWCC1 is upregulated in hypoxia, a pathological condition characterized by an inadequate supply of oxygen in the blood.

Function:
May act as a transcriptional repressor. Down-regulates CA9 expression.

Subunit:
Interacts with HDAC4.

Subcellular Location:
Nucleus. Cytoplasm, cytosol. Note=Mainly located in the nucleus.

Tissue Specificity:
Highly expressed in smooth muscle, pancreas and testis.

Similarity:
Contains 1 CW-type zinc finger.

SWISS:
Q9Y6X9

Gene ID:
22880

Database links:

Entrez Gene: 22880 Human

Entrez Gene: 74522 Mouse

Entrez Gene: 289736 Rat

SwissProt: Q9Y6X9 Human

Unigene: 143840 Human

Unigene: 555918 Human

Unigene: 139127 Mouse



產品圖片
Sample: MCF-7(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- phospho-ZCWCC1 (Ser739) (bs-10016R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ZCWCC1 (Ser739)) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-ZCWCC1(Ser739) Polyclonal Antibody, Unconjugated(bs-10016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-ZCWCC1 (Ser739)) polyclonal Antibody, Unconjugated (bs-10016R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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