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ST18 Rabbit pAb (bs-12188R)  
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產品編號 bs-12188R
英文名稱 ST18 Rabbit pAb
中文名稱 腫瘤抑制蛋白18抗體
別    名 KIAA0535; St18; ST18_HUMAN; suppression of tumorigenicity 18(breast carcinoma)(zinc finger protein); Suppression of tumorigenicity 18 protein; Suppression of tumorigenicity protein 18; Zinc finger protein 387; Znf387.  
研究領域 腫瘤  細胞生物  干細胞  鋅指蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Sheep,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 115 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ST18/Znf387: 451-550/1047 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 ST18 is a 1,047 amino acid nuclear protein that is expressed at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Containing six C2HC-type zinc fingers and an SMC domain, ST18 is a repressor protein that binds to DNA sequences containing a specific bipartite element. ST18 is considered a transcription factor that represses basal transcription activity from target promoters and Inhibits colony formation in cultured breast cancer cells. ST18 is encoded by a gene located on human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Function:
Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.

Subcellular Location:
Nucleus.

Tissue Specificity:
Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells.

Similarity:
Contains 6 C2HC-type zinc fingers.

SWISS:
O60284

Gene ID:
9705

Database links:

Entrez Gene: 9705 Human

Entrez Gene: 240690 Mouse

Entrez Gene: 266680 Rat

SwissProt: O60284 Human

SwissProt: Q80TY4 Mouse

SwissProt: Q9QX27 Rat

Unigene: 655499 Human

Unigene: 234612 Mouse

Unigene: 91311 Rat



產品圖片
Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- ST18 (bs-12188R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 110 kD
Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- ST18 (bs-12188R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ST18 Polyclonal Antibody, Unconjugated(bs-12188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-ST18 antibody (bs-12188R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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