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NR5A2 Rabbit pAb (bs-10444R)  
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產品編號 bs-10444R
英文名稱 NR5A2 Rabbit pAb
中文名稱 成纖維細胞核受體Nr5a2抗體
別    名 NR5A2/LRH1; alpha-1-fetoprotein transcription factor; B1-binding factor; B1F; B1F2; CPF; CYP7A promoter-binding factor; FTF; FTZ F1; FTZ F1beta; FTZ-F1; FTZ-F1beta; hB1F 2; HB1F; hB1F-2; Hepatocytic transcription factor; Liver receptor homolog 1; LRH-1; LRH1; Nr5a2; NR5A2_HUMAN; Nuclear receptor subfamily 5 group A member 2.  
研究領域 細胞生物  發育生物學  染色質和核信號  微生物學  信號轉導  干細胞  轉錄調節因子  細菌及病毒  細胞表面分子  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Cow,Chicken)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 61 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NR5A2/LRH1: 151-250/541 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 NR5A2 is a pre-adipocyte-specific nuclear receptor that regulates expression of aromatase in adipose tissue. NR5A2 belongs to the fushi tarazu factor-1 subfamily of orphan nuclear receptors. NR5A2 transcripts are abundant in the human ovary and testis and are predominantly expressed in tissues of endodermal origin. NR5A2 is a positive transcription factor for ABCG5 and ABCG8 and regulates genes involved in sterol and bile acid secretion from liver and intestine. It induces cell proliferation through the concomitant induction of cyclin D1 and E1, an effect that is potentiated by its interaction with beta-catenin.

Function:
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

Subcellular Location:
Nucleus.

Tissue Specificity:
Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in hepG2. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and hepG2 cells.

Similarity:
Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
O00482

Gene ID:
2494

Database links:

Entrez Gene: 541305 Cow

Entrez Gene: 2494 Human

Entrez Gene: 26424 Mouse

Entrez Gene: 60349 Rat

Omim: 604453 Human

SwissProt: O00482 Human

SwissProt: P45448 Mouse

SwissProt: Q9QWM1 Rat

Unigene: 33446 Human

Unigene: 16794 Mouse

Unigene: 42941 Rat



產品圖片
Sample: Liver(Rat) Lysate at 40 ug Primary: Anti-NR5A2 (bs-10444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR5A2 Polyclonal Antibody, Unconjugated(bs-10444R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR5A2) Polyclonal Antibody, Unconjugated (bs-10444R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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