麻豆av资源_日本三级一区_www.狠狠艹_国产精妇在线观看第一区_成人性生交大片免费看中国A片_日本一本久久

掃碼關(guān)注公眾號           掃碼咨詢技術(shù)支持           掃碼咨詢技術(shù)服務(wù)
  
客服熱線:400-901-9800  客服QQ:4009019800  技術(shù)答疑  技術(shù)支持  質(zhì)量反饋  關(guān)于我們  聯(lián)系我們
88mav在线播放,欧美另类综合,免费性av
首頁 > 產(chǎn)品中心 > 一抗 > 產(chǎn)品信息
ASCL2 Rabbit pAb (bs-12349R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術(shù)支持:techsupport@bioss.com.cn
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-12349R
英文名稱 ASCL2 Rabbit pAb
中文名稱 ASCL2蛋白抗體
別    名 Achaete scute complex like 2; Achaete scute homolog 2; achaete-scute complex-like 2; Achaete-scute homolog 2; ASCL2; ASCL2_HUMAN; ASH-2; Ash2; bHLHa45; Class A basic helix-loop-helix protein 45; HASH2; Mash2.  
Specific References  (4)     |     bs-12349R has been referenced in 4 publications.
[IF=22.387] Bu P et al. A miR-34a-Numb Feedforward Loop Triggered by Inflammation Regulates Asymmetric Stem Cell Division in Intestine and Colon Cancer. (2016) Cell.Stem.Cell. 18:189-202  IF ;  Human&Mouse.  
[IF=8.786] Lei Wu. et al. ASCL2 Affects the Efficacy of Immunotherapy in Colon Adenocarcinoma Based on Single-Cell RNA Sequencing Analysis. FRONT IMMUNOL. 2022; 13: 829640  IHC ;  Human.  
[IF=6.244] Zhang W. et al. Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer.. Front Oncol. 2022 Mar;12:805290-805290  IHC ;  Human.  
[IF=5.108] Mitkin NA et al. Protective C allele of the single-nucleotide polymorphism rs1335532 is associated with strong binding of Ascl2 transcription factor and elevated CD58 expression in B-cells.Biochim Biophys Acta Mol Basis Dis. 2018 Oct;1864(10):3211-3220.  Pull-Down Assay ;  Human.  
研究領(lǐng)域 細(xì)胞生物  發(fā)育生物學(xué)  信號轉(zhuǎn)導(dǎo)  干細(xì)胞  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse (predicted: Rat,Pig,Cow,Chicken,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Human ASCL2: 51-120/193 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (BETA2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix (bHLH) transcription factors that function as dominant negative regulators and are involved in cell lineage determination and differentiation. ASCL2 is a 193 amino acid protein that localizes to the nucleus and contains one bHLH domain. Expressed in developing placental tissue, ASCL2 binds to DNA and functions as a transcriptional regulator that is involved in the maturation of neuronal precursors in the peripheral and central nervous systems.

Function:
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed specifically in the extravillous trophoblasts of the developing placenta.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:
Q99929

Gene ID:
430

Database links:

Entrez Gene: 430 Human

Entrez Gene: 17173 Mouse

Entrez Gene: 24209 Rat

SwissProt: Q99929 Human

SwissProt: O35885 Mouse

SwissProt: P19360 Rat

Unigene: 152475 Human

Unigene: 196417 Mouse

Unigene: 10486 Rat



產(chǎn)品圖片
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated(bs-12349R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated(bs-12349R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated(bs-12349R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ASCL2) polyclonal Antibody, Unconjugated (bs-12349R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:K562. Primary Antibody (green line): Rabbit Anti-ASCL2 antibody (bs-12349R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版權(quán)所有 2004-2026 m.jywcc.com 北京博奧森生物技術(shù)有限公司
通過國際質(zhì)量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫(yī)療器械-質(zhì)量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網(wǎng)安備110107000727號
主站蜘蛛池模板: 久久精品性视频 | av一区二区三区四区 | 日韩一区二区三区北条麻妃 | 精品h视频| 亚洲久在线| 女高中生边自慰边呻吟 | 国产成年人黄色视频 | 中文字幕日本在线观看 | 免费看麻豆 | 亚洲成人av一区 | 国产美女裸体永久无遮挡 | 国产成人精品视频一区二区不卡 | 中国丰满熟妇AV | 乱一色一毛一片 | 在线观看免费a视频 | 婷婷六月色 | 69堂在线| qvod视频在线观看成人 | 97超碰caoporen公开人人爽 | 欧美一区二区三区爽大粗免费 | www.日本免费 | 免费观看日本污污ww网站 | 欧类av怡春院 | 国产精品色婷婷在线观看 | 天天日本天天谢 | 国产亚洲综合AA系列 | 欧美天天澡天天爽日日A | 欧美日本国产VA高清CABAL | 91网站在线观看免费 | 韩国中文免费在线视频 | 欧美一区二区三区免费 | 精品久久久久久国产牛牛 | 夜趣福利视频 | 亚洲国产另类久久久精品小说 | 刘亦菲一区二区三区免费看 | 艹逼大片 | 国产日韩欧美在线一区 | 午夜在线不卡精品国产 | 国内精品免费久久久久软件老师 | 91在线精品一区二区 | 国产精品中文久久久久久久 |