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DBPA Rabbit pAb (bs-12985R)  
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產(chǎn)品編號(hào) bs-12985R
英文名稱 DBPA Rabbit pAb
中文名稱 冷休克蛋白DBPA抗體
別    名 Cold shock domain containing protein A; Cold shock domain-containing protein A; csdA; CSDA1; DBPA; DNA binding protein A; DNA-binding protein A; Single strand DNA binding protein NF GMB; Single-strand DNA-binding protein NF-GMB; ZO 1 associated nucleic acid binding protein; ZO1 associated nucleic acid binding protein; ZONAB; YBOX3_HUMAN; Y-box-binding protein 3.  
Specific References  (1)     |     bs-12985R has been referenced in 1 publications.
[IF=6.706] Je?ena Moisejenko-Golubovi?a. et al. Serum Vitamin D Levels Explored in the Latvian Cohort of Patients with Basal Cell Carcinoma Linked to the Sonic Hedgehog and Vitamin D Binding Protein Cutaneous Tissue Indices. NUTRIENTS. 2022 Jan;14(16):3359  IHC ;  Human.  
研究領(lǐng)域 細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞周期蛋白  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 40 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DBPA/CSDA1: 165-270/372 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CSDA is a 372 amino acid nuclear and cytoplasmic protein that is highly expressed in skeletal muscle and heart. Containing one CSD (cold-shock) domain, CSDA is thought to bind to GM-CSF promoter, full length mRNA and to short RNA sequences containing a specific consensus site. CSDA is suggested to have a role in translation repression and is found in a mRNP complex with MSY2. MSY2 belongs to the Y-box family of multifunctional proteins that regulate both transcription and translation. CSDA participates in promoting cell proliferation and expression of cyclin D1 and proliferating cell nuclear antigen (PCNA). CSDA is regarded to be an important component of the mechanisms that sense epithelial density and in regulating the switch between proliferation and differentiation through complex transcriptional networks.

Function:
Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Highly expressed in skeletal muscle and heart.

Similarity:
Contains 1 CSD (cold-shock) domain.

SWISS:
P16989

Gene ID:
8531

Database links:

Entrez Gene: 8531 Human

Entrez Gene: 56449 Mouse

Entrez Gene: 83807 Rat

Omim: 603437 Human

SwissProt: P16989 Human

SwissProt: Q61478 Mouse

SwissProt: Q68G78 Mouse

SwissProt: Q8BPG0 Mouse

SwissProt: Q9JKB3 Mouse

SwissProt: Q3KRC7 Rat

SwissProt: Q62764 Rat

Unigene: 221889 Human

Unigene: 299604 Mouse

Unigene: 458000 Mouse

Unigene: 3306 Rat



產(chǎn)品圖片
Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Rat Muscle tissue lysates Primary: Anti-DBPA (bs-12985R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DBPA) Polyclonal Antibody, Unconjugated (bs-12985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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