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FOXRED2 Rabbit pAb (bs-13210R)  
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產品編號 bs-13210R
英文名稱 FOXRED2 Rabbit pAb
中文名稱 FOXRED2蛋白抗體
別    名 D15Bwg0759e; Endoplasmic reticulum flavoprotein associated with degradation; ERFAD; FAD dependent oxidoreductase domain containing 2; FAD-dependent oxidoreductase domain-containing protein 2; FLJ23322; foxred2; FXRD2_HUMAN; RP5-1119A7.4.  
研究領域 腫瘤  細胞生物  神經生物學  信號轉導  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 75 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FOXRED2: 21-120/684 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 FOXRED2 is a 684 amino acid protein that exists as multiple alternatively spliced isoforms. The gene encoding FOXRED2 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. Additionally, translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein BCR-Abl, a potent cell proliferation activator found in several types of leukemias.

Function:
Probable flavoprotein which may function in endoplasmic reticulum associated degradation (ERAD). May bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation.

Subunit:
Interacts with SEL1L. May interact with OS9 and DNAJC10.

Subcellular Location:
Endoplasmic reticulum lumen.

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the FOXRED2 family.

SWISS:
Q8IWF2

Gene ID:
80020

Database links:

Entrez Gene: 80020 Human

Omim: 613777 Human

SwissProt: Q8IWF2 Human

Unigene: 387601 Human



產品圖片
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FOXRED2 Polyclonal Antibody, Unconjugated(bs-13210R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FOXRED2 Polyclonal Antibody, Unconjugated(bs-13210R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Mouse kidney. Primary Antibody (green line): Rabbit Anti-FOXRED2 antibody (bs-13210R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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