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KEAP1 Rabbit pAb (bs-4900R)  
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產品編號 bs-4900R
英文名稱 KEAP1 Rabbit pAb
中文名稱 胞質接頭蛋白Keap1
別    名 Cytosolic inhibitor of Nrf2; INrf 2; INrf2; Keap 1; KEAP1; KEAP1_HUMAN; Kelch like ECH associated protein 1; Kelch like protein 19; Kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132; KLHL 19; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454.  
Specific References  (11)     |     bs-4900R has been referenced in 11 publications.
[IF=7.129] Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546  WB ;  Chicken.  
[IF=4.571] Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297  WB ;  Rat.  
[IF=4.253] Mengru Li. et al. The leaves of Scutellaria baicalensis Georgi attenuate brain aging in D-galactose-induced rats via regulating glutamate metabolism and Nrf2 signaling pathway. EXP GERONTOL. 2022 Dec;170:111978  WB ;  Rat.  
[IF=4.155] Quanwei Li. et al. Toxicological mechanism of large amount of copper supplementation: Effects on endoplasmic reticulum stress and mitochondria-mediated apoptosis by Nrf2/HO-1 pathway-induced oxidative stress in the porcine myocardium. J Inorg Biochem. 2022 May;230:111750  WB ;  Pig.  
[IF=4.125] Hong Zhao. et al. Polysaccharides from sea buckthorn (Hippophae rhamnoides L.) berries ameliorates cognitive dysfunction in AD mice induced by a combination of D-gal and AlCl3 by suppressing oxidative stress and inflammation reaction. J SCI FOOD AGR. 2023 May;:  WB ;  Mouse.  
[IF=3.943] Kaiqiang Fu. et al. Berberine attenuates the inflammatory response by activating the Keap1/Nrf2 signaling pathway in bovine endometrial epithelial cells. Int Immunopharmacol. 2021 Jul;96:107738  WB,IF ;  Bovine.  
[IF=3.738] Rao, Gan. et al. Arsenic Trioxide Triggers Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis via Nrf 2/Caspase 3 Signaling Pathway in Heart of Ducks. BIOL TRACE ELEM RES. Biol Trace Elem Res. 2022 Apr;:1-11  WB ;  Duck.  
[IF=3.738] Chen, Bo. et al. Acute Aluminum Sulfate Triggers Inflammation and Oxidative Stress, Inducing Tissue Damage in the Kidney of the Chick. BIOL TRACE ELEM RES. 2022 May;:1-9  WB ;  Chicken.  
[IF=3.448]   
[IF=1.41] Yi X et al. Expression of Keap1 and Nrf2 in diffuse large B?cell lymphoma and its clinical significance.Exp Ther Med. 2018 Aug;16(2):573-578.  IHC-P ;  Human.  
[IF=0.5] Shanshan Qi. et al. The hepatoprotective effect of Sophora viciifolia fruit extract against acetaminophen-induced liver injury in mice. J APPL BIOMED. 2023 May 18  IHC ;  Mouse.  
研究領域 腫瘤  細胞生物  發育生物學  信號轉導  細胞凋亡  轉錄調節因子  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 69 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KEAP1: 21-120/624 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

Subunit:
Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1.

Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus.

Tissue Specificity:
Broadly expressed, with highest levels in skeletal muscle.

Similarity:
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.

SWISS:
Q14145

Gene ID:
9817

Database links:

Entrez Gene: 532791 Cow

Entrez Gene: 476713 Dog

Entrez Gene: 9817 Human

Entrez Gene: 50868 Mouse

Entrez Gene: 100136900 Pig

Entrez Gene: 117519 Rat

Entrez Gene: 321837 Zebrafish

Omim: 606016 Human

SwissProt: Q14145 Human

SwissProt: Q9Z2X8 Mouse

SwissProt: Q684M4 Pig

SwissProt: P57790 Rat

Unigene: 465870 Human

Unigene: 248266 Mouse

Unigene: 23467 Rat



產品圖片
Sample: A673(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-KEAP1 (bs-4900R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-4900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
A-549cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KEAP1) polyclonal Antibody, Unconjugated (bs-4900R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A-431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KEAP1) polyclonal Antibody, Unconjugated (bs-4900R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control(black line):A549. Primary Antibody (green line): Rabbit Anti-KEAP1 antibody (bs-4900R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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