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SIT Rabbit pAb (bs-13701R)  
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產品編號 bs-13701R
英文名稱 SIT Rabbit pAb
中文名稱 跨膜接頭蛋白SIT抗體
別    名 gp30/40; SHP intracting transmembrane adapter protein; SHP2-interacting transmembrane adapter protein; Signaling threshold-regulating transmembrane adapter 1; SIT; Sit1; SIT1_HUMAN; Suppression inducing transmembrane adapter 1; Suppression-inducing transmembrane adapter 1.  
研究領域 細胞生物  信號轉導  淋巴細胞  t-淋巴細胞  b-淋巴細胞  跨膜蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Pig,Sheep,Cow)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 18 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SIT: 51-150/196 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Function:
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.

Post-translational modifications:
Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.

SWISS:
Q9Y3P8

Gene ID:
27240

Database links:

Entrez Gene: 27240 Human

Entrez Gene: 54390 Mouse

Omim: 604964 Human

SwissProt: Q9Y3P8 Human

SwissProt: Q8C503 Mouse

Unigene: 88012 Human

Unigene: 31775 Mouse



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIT) Polyclonal Antibody, Unconjugated (bs-13701R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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