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CALB1 Rabbit pAb (bs-10177R)  
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產品編號 bs-10177R
英文名稱 CALB1 Rabbit pAb
中文名稱 鈣結合蛋白D28K抗體
別    名 CALB1_HUMAN; Calbindin; CALB1; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CAB27; Calbindin-D28k;  
研究領域 細胞生物  免疫學  神經生物學  生長因子和激素  結合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29 kDa
檢測分子量
細胞定位 細胞核 細胞漿 細胞外基質 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Calbindin: 41-150/261 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]

Function:
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Subunit:
Interacts with RANBP9.

Similarity:
Belongs to the calbindin family.
Contains 5 EF-hand domains.

SWISS:
P05937

Gene ID:
793

Database links:

Entrez Gene: 793 Human

Entrez Gene: 12307 Mouse

Entrez Gene: 83839 Rat

SwissProt: P05937 Human

SwissProt: P12658 Mouse

SwissProt: P07171 Rat



產品圖片
Sample: kidney (mouse) Lysate at 40 ug brain (Mouse) Lysate at 40 ug Primary: Anti-Calbindin(bs-10177R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 29 kD Observed band size: 29 kD
Sample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Mouse Kidney Lysates Lane 4: Rat Kidney Lysates Primary: Anti-Calbindin (bs-10177R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29kDa Observed band size: 29kDa
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin ) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
4% Paraformaldehyde-fixed SHSY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Calbindin) polyclonal Antibody, unconjugated (bs-10177R) 1:50, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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