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NeuN Rabbit pAb (bs-10394R)  
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產品編號 bs-10394R
英文名稱 NeuN Rabbit pAb
中文名稱 神經元核抗原抗體
別    名 RFOX3_HUMAN; RNA binding protein fox-1 homolog 3; RBFOX3; Fox-1 homolog C; Neuronal nuclei antigen(NeuN antigen); FOX3; FOX-3; HRNBP3;  
Specific References  (8)     |     bs-10394R has been referenced in 8 publications.
[IF=9.038] Zhi Li. et al. Long-term exposure to 2-amino-3-methylimidazo[4,5-f]quinoline can trigger a potential risk of Parkinson's disease. J Hazard Mater. 2021 Jun;412:125230  IF ;  Zebrafish.  
[IF=6.291] Hongmei Zhou. et al. Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain. Ecotox Environ Safe. 2022 Jan;229:113066  IF ;  Mouse.  
[IF=6.208] Jia Wang. et al. FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice. INT J MOL SCI. 2022 Jan;23(23):14979  IHC, IF ;  Mouse.  
[IF=4.831] Haoqiang Zhang. et al. Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation.. Aging-Us. 2021 Jan 15; 13(1): 525–536  IF ;  Fish.  
[IF=4.652] Zhang et al. The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats. (2016) Cell.Physiol.Bioche. 39:1152-64  IHC-P ;  Rat.  
[IF=4.398] Danyang Li. et al. Heterozygous disruption of beclin 1 alleviates neurotoxicity induced by sub-chronic exposure of arsenite in mice. NEUROTOXICOLOGY. 2022 Oct;:  IHC ;  Mouse.  
[IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.  IF ;  Mouse.  
[IF=2.784] Xiong?LL et al. Tree shrew neural stem cell transplantation promotes functional recovery of tree shrews with a hemi?sectioned spinal cord injury by upregulating nerve growth factor expression. Int J Mol Med. 2018 Jun;41(6):3267-3277.  IF ;  tree shrews.  
研究領域 細胞生物  神經生物學  細胞凋亡  轉運蛋白  細胞類型標志物  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat (predicted: Mouse,Sheep,Cow,Bee,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 34 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FOX3: 101-200/312 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Function:
RNA-binding protein that regulates alternative splicing events.

Subcellular Location:
Nucleus. Cytoplasm.

Tissue Specificity:
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
A6NFN3

Gene ID:
146713

Database links:

Entrez Gene: 146713 Human

Entrez Gene: 52897 Mouse

Entrez Gene: 287847 Rat

SwissProt: A6NFN3 Human

SwissProt: Q8BIF2 Mouse

Unigene: 135229 Human

Unigene: 341103 Mouse

Unigene: 143966 Rat



產品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NeuN) polyclonal Antibody, Unconjugated (bs-10394R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:A549. Primary Antibody (green line): Rabbit Anti-NeuN antibody (bs-10394R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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