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DOC2B Rabbit pAb (bs-14402R)  
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產品編號 bs-14402R
英文名稱 DOC2B Rabbit pAb
中文名稱 DOC2B蛋白抗體
別    名 Doc2 beta; Doc2-beta; Doc2b; DOC2B_HUMAN; DOC2BL; Double C2 like domain containing protein beta; Double C2 like domains beta; Double C2 like domains beta like; Double C2-like domain-containing protein beta; OTTMUSP00000006522; RP23 78H4.2.  
研究領域 細胞生物  神經生物學  信號轉導  轉運蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Sheep,Cow,Chicken,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 46 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DOC2B: 281-380/412 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq, Jul 2008]

Function:
Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes (By similarity).

Subcellular Location:
Cytoplasm. Cytoplasmic granule. Cell membrane. Translocates to the plasma membrane in a calcium-dependent manner.

Tissue Specificity:
Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level).

Similarity:
Contains 2 C2 domains.

SWISS:
Q14184

Gene ID:
8447

Database links:

Entrez Gene: 8447 Human

Entrez Gene: 13447 Mouse

Entrez Gene: 81820 Rat

Omim: 604568 Human

SwissProt: Q14184 Human

SwissProt: P70169 Mouse

SwissProt: P70610 Rat

Unigene: 551705 Human

Unigene: 5137 Mouse

Unigene: 10689 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOC2B)) Polyclonal Antibody, Unconjugated (bs-14402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOC2B)) Polyclonal Antibody, Unconjugated (bs-14402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOC2B) Polyclonal Antibody, Unconjugated (bs-14402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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