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ZFYVE26 Rabbit pAb (bs-19159R)  
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產(chǎn)品編號 bs-19159R
英文名稱 ZFYVE26 Rabbit pAb
中文名稱 鋅指蛋白結(jié)構(gòu)域ZFYVE26抗體
別    名 DKFZp686F19106; DKFZp781H1112; FYVE domain-containing centrosomal protein; FYVE-CENT; KIAA0321; Spastic paraplegia 15(complicated autosomal recessive); Spastizin; SPG15; ZFY26_HUMAN; ZFYVE 26; ZFYVE26; Zinc finger FYVE domain containing 26; Zinc finger FYVE domain containing protein 26; Zinc finger FYVE domain-containing protein 26.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  鋅指蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 285 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ZFYVE26: 2151-2250/2539 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein which contains a FYVE zinc finger binding domain. The presence of this domain is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. [provided by RefSeq, Oct 2008]

Function:
Phosphatidylinositol 3-phosphate-binding protein required for the abcission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abcission. May also be required for efficient homologous recombination DNA double-strand break repair.

Subcellular Location:
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. Localizes to the centrosome during all stages of the cell cycle. Recruited to the midbody during cytokinesis by KIF13A.

Tissue Specificity:
Strongest expression in the adrenal gland, bone marrow, adult brain, fetal brain, lung, placenta, prostate, skeletal muscle, testis, thymus, and retina. Intermediate levels are detected in other structures, including the spinal cord.

DISEASE:
Spastic paraplegia 15, autosomal recessive

Similarity:
Contains 1 FYVE-type zinc finger.

SWISS:
Q68DK2

Gene ID:
23503

Database links:

Entrez Gene: 514402 Cow

Entrez Gene: 23503 Human

Entrez Gene: 211978 Mouse

Entrez Gene: 314265 Rat

Omim: 612012 Human

SwissProt: E1BLZ4 Cow

SwissProt: Q68DK2 Human

SwissProt: Q5DU37 Mouse

SwissProt: D4A8G9 Rat

Unigene: 98041 Human

Unigene: 297919 Mouse

Unigene: 16048 Rat



產(chǎn)品圖片
Sample: Brain (Mouse) Lysate at 40 ug U251 (Human) CellLysate at 30 ug U87MG (Human) CellLysate at 30 ug Primary: Anti-ZFYVE26 (bs-19159R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 285 kD Observed band size: 285 kD
Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-ZFYVE26 (bs-19159R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 285 kD Observed band size: 285 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFYVE26) Polyclonal Antibody, Unconjugated (bs-19159R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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