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NGFB Rabbit pAb (bs-10806R)  
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產品編號 bs-10806R
英文名稱 NGFB Rabbit pAb
中文名稱 神經生長因子β抗體
別    名 NGF; beta nerve growth factor; NGF-beta; beta nerve growth factor precursor; beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF B; NGFB; NID67; NGF beta; NGF-B; NGF_HUMAN.  
Specific References  (1)     |     bs-10806R has been referenced in 1 publications.
[IF=1.894] Zhou M et al. Cardiac Sympathetic Afferent Denervation Protects Against Ventricular Arrhythmias by Modulating Cardiac Sympathetic Nerve Activity During Acute Myocardial Infarction.Med Sci Monit. 2019 Mar 16;25:1984-1993.  WB ;  Dog.  
研究領域 發育生物學  神經生物學  細胞因子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 13/32 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinded human NGF beta full length protein 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Function:
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

DISEASE:
Defects in NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [MIM:608654]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.

Similarity:
Belongs to the NGF-beta family.

SWISS:
P01138

Gene ID:
4803

Database links:

Entrez Gene: 4803 Human

Entrez Gene: 18049 Mouse

Entrez Gene: 310738 Rat

Omim: 162030 Human

SwissProt: P01138 Human

SwissProt: P01139 Mouse

SwissProt: P25427 Rat

Unigene: 2561 Human

Unigene: 1259 Mouse

Unigene: 22168 Rat



產品圖片
Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skin (Mouse) Lysate at 40 ug Lane 3: Thyroid gland (Mouse) Lysate at 40 ug Primary: Anti-NGFB (bs-10806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32/13 kD Observed band size: 35/13 kD
Sample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Human U-87 MG cell Lysates Lane 4: Human SH-SY5Y cell Lysates Lane 5: Human U-2 OS cell Lysates Primary: Anti-NGFB? (bs-10806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/32kDa Observed band size: 16/35kDa
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NGFB Polyclonal Antibody, Unconjugated(bs-10806R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (bs-10806R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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