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PSMC3 Rabbit pAb (bs-19463R)  
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產品編號 bs-19463R
英文名稱 PSMC3 Rabbit pAb
中文名稱 蛋白酶體26S亞基ATP酶3抗體
別    名 26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Human immunodeficiency virus tat transactivator binding protein 1; MGC8487; Proteasome(prosome macropain) 26S subunit ATPase 3; Proteasome 26S ATPase subunit 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; PRS6A_HUMAN; PSMC 3; PSMC3; Tat binding protein 1; TAT-binding protein 1; TBP-1; TBP1.  
研究領域 細胞生物  結合蛋白  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse (predicted: Cow,Dog,Horse,Xenopus laevis)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 49 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PSMC3: 212-220/439 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]

Function:
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.

Subcellular Location:
Cytoplasm. Nucleus.

Post-translational modifications:
Sumoylated by UBE2I in response to MEKK1-mediated stimuli.

Similarity:
Belongs to the AAA ATPase family.

SWISS:
P17980

Gene ID:
5702

Database links:

Entrez Gene: 5702 Human

Entrez Gene: 19182 Mouse

Entrez Gene: 29677 Rat

Entrez Gene: 379370 Xenopus laevis

Omim: 186852 Human

SwissProt: P17980 Human

SwissProt: O88685 Mouse

SwissProt: Q63569 Rat

SwissProt: O42586 Xenopus laevis

Unigene: 250758 Human

Unigene: 289832 Mouse

Unigene: 11173 Rat



產品圖片
Sample: Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-PSMC3 (bs-19463R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMC3) Polyclonal Antibody, Unconjugated (bs-19463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMC3) Polyclonal Antibody, Unconjugated (bs-19463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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