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MBP tag Mouse mAb (bsm-33008M)  
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50ul/680.00元
100ul/980.00元
500ul/4000.00元
200ug(PBS only)/5600.00元
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產品編號 bsm-33008M
英文名稱 MBP tag Mouse mAb
中文名稱 麥芽糖結合蛋白單克隆抗體
別    名 MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI.  
Specific References  (1)     |     bsm-33008M has been referenced in 1 publications.
[IF=3.197] Wei Lu. et al. Molecular mechanisms by which targeted muscle reinnervation improves the microenvironment of spinal cord motor neurons and target muscles. NEUROSCI LETT. 2022 Oct;789:136879  IHC ;  Rat.  
產品類型 標簽抗體 
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 9G2
交叉反應 Recombinant protein
產品應用 WB=1:1000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 44 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant MBP Protein of Escherichia coli (strain K12) 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Function:
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.

Database links:
Entrez Gene: 1039564 Escherichia coli

Entrez Gene: 4494002 Escherichia coli

Entrez Gene: 948538 Escherichia coli

Entrez Gene: 960089 Escherichia coli

SwissProt: P0AEX9 Escherichia coli

SwissProt: P0AEY0 Escherichia coli



產品圖片
100 ng rhC-jun-His-MBP protein (bs-42050P) per lane probed with MBP tag monoclonal antibody respectively, unconjugated (bsm-33008M) at 1:10000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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