麻豆av资源_日本三级一区_www.狠狠艹_国产精妇在线观看第一区_成人性生交大片免费看中国A片_日本一本久久

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質量反饋  關于我們  聯系我們
男人女人做爽爽18禁免费,亚洲AV无码之国产精品,国产乱插
首頁 > 產品中心 > 一抗 > 產品信息
Cyclin E1 Recombinant Rabbit mAb (bsm-52048R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
50ul/1400.00元
100ul/2500.00元
大包裝/詢價

產品編號 bsm-52048R
英文名稱 Cyclin E1 Recombinant Rabbit mAb
中文名稱 周期素E重組兔單抗
別    名 CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1.  
Specific References  (1)     |     bsm-52048R has been referenced in 1 publications.
[IF=3.738] Qiao Zhang. et al. G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression. Int J Med Sci. 2022; 19(1): 47–64  IHC ;  Human.  
研究領域 細胞生物  細胞周期蛋白  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 4H7
交叉反應 Human,Mouse
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 A synthesized peptide derived from human Cyclin E1: 350-410 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Function:
Essential for the control of the cell cycle at the G1/S (start) transition.

Subunit:
Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.

Subcellular Location:
Nucleus.

Tissue Specificity:
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Post-translational modifications:
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the cyclin family. Cyclin E subfamily.

SWISS:
P24864

Gene ID:
898

Database links:

Entrez Gene: 898 Human

Entrez Gene: 12447 Mouse

Entrez Gene: 25729 Rat

Omim: 123837 Human

SwissProt: P24864 Human

SwissProt: Q61457 Mouse

SwissProt: P39949 Rat

Unigene: 244723 Human

Unigene: 16110 Mouse

Unigene: 15455 Rat



產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Cyclin E1 monoclonal antibody, unconjugated (bsm-52048R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin E1) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin E1) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin E1) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
版權所有 2004-2026 m.jywcc.com 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號
主站蜘蛛池模板: 女警一级毛片免费视频观看 | 乱码国产丰满人妻WWW | 国产精品全新69影院在线看 | 欧美亚洲日本国产综合在线 | 久久99精品久久久久久清纯 | 久久伊人天堂 | 蝴蝶中文综合娱乐网 | 日韩在线综合 | 久久99国产综合精合精品 | 午夜国产情侣在线播放 | 精品多毛少妇人妻AV免费久久 | 少妇看A片偷人精品视频 | 99亚洲男女激情在线观看 | 成人午夜精品无码一区二区三区 | 99中文在线 | 老头巨大挺进莹莹的体内免费视频 | 美女一级 | 黄色天堂在线观看 | 老熟女高潮喷了一地 | 亚洲一区二区三区免费在线观看 | 久久国产精品综合 | 亚洲性在线 | 欧美日韩免费在线观看 | 七七婷婷婷婷精品国产 | 免费a级毛片又大又粗又黑 秋霞久久久久久一区二区 久久亚洲精品国产 | 国产精品www劲爽视频 | 18禁裸男晨勃露J毛免费观看 | 亚洲看片网 | 18禁无码无遮挡h动漫免费看 | 古惑仔1人在江湖在线观看粤语高清 | 性盈盈影院67194免费影隐 | 欧美囗交xx×bbb视频 | 天天天天噜在线视频 | 亚洲免费黄色网址 | 在线免费毛片视频 | 婷婷影院在线综合免费视频 | 中文人妻精品一区在线 | 国产丝袜av一区二区三区性色 | heyzo无码中文字幕在线 | 欧美日韩色综合 | 久久99精品久久久秒播 |