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Pan Cytokeratin(ready to use) Mouse mAb (BH0149)  
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3ml/560.00元
6ml/980.00元
大包裝/詢價

產品編號 BH0149
英文名稱 Pan Cytokeratin(ready to use) Mouse mAb
中文名稱 廣譜細胞角蛋白PCK單克隆抗體
別    名 pan-cytokeratin; pan-CK; pan CK; P-CK; CK-PAN; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1, 2, 4, 5, 6, 7, 8, 71, 72, 75, 78].  
Specific References  (1)     |     BH0149 has been referenced in 1 publications.
[IF=3.998] Ma Z et al. Characterisation of a subpopulation of CD133 + cancer stem cells from Chinese patients with oral squamous cell carcinoma. Sci Rep. 2020 Jun 1;10(1):8875.  IHC-P ;  Human.  
研究領域 腫瘤  細胞生物  免疫學  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 4C3
交叉反應 Human
產品應用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 42-64 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Pan Cytokeratin 
亞    型 IgG2b
純化方法 affinity purified by Protein G
緩 沖 液 0.01M PBS (pH7.4) with 1% BSA and 0.02% Proclin300.
保存條件 Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.

Subcellular Location:
Cytoplasmic.

Tissue Specificity:
epithelial cells

SWISS:
Q2M2I5

Gene ID:
192666

產品圖片
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Monoclonal Antibody, Unconjugated (BH0149) overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Monoclonal Antibody, Unconjugated (BH0149) overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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