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TNFR2 Recombinant Rabbit mAb (bsm-52938R)  
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產品編號 bsm-52938R
英文名稱 TNFR2 Recombinant Rabbit mAb
中文名稱 腫瘤壞死因子受體2重組兔單抗
別    名 TNF Receptor II; CD120b; p75; p75TNFR; p80 TNF alpha receptor; p80 TNF-alpha receptor; TBP-2; TBPII; TNF R2; TNF R75; TNF-R2; TNF-RII; TNFR-II; TNFR80; TNFRII; TNFRSF1B; TNR1B_HUMAN; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2.  
研究領域 腫瘤  細胞生物  神經生物學  信號轉導  細胞凋亡  細胞膜受體  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 7D3
交叉反應 Human (predicted: Mouse,Rat)
產品應用 IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 46 kDa
檢測分子量
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 A synthesized peptide derived from human TNFRSF1B: 415-461/461 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Function:
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Subcellular Location:
Secreted and Cell membrane.

Post-translational modifications:
Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

Similarity:
Contains 4 TNFR-Cys repeats.

SWISS:
P20333

Gene ID:
7133

Database links:

Entrez Gene: 7133 Human

Entrez Gene: 21938 Mouse

Entrez Gene: 156767 Rat

Omim: 191191 Human

SwissProt: P20333 Human

SwissProt: P25119 Mouse

SwissProt: Q80WY6 Rat

Unigene: 256278 Human

Unigene: 235328 Mouse

Unigene: 83633 Rat



產品圖片
Sample: Lane 1: Human THP-1 cell Lysates Lane 2: Human K562 cell Lysates Lane 3: Human U937 cell Lysates Lane 4: Human U87MG cell Lysates Lane 5: Human HUVEC cell Lysates Primary: Anti-TNFR2 (bsm-52938R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46kDa Observed band size: 70 kDa
Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (bsm-52938R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (bsm-52938R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (bsm-52938R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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