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NRF1 Recombinant Rabbit mAb (bsm-54140R)  
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產(chǎn)品編號 bsm-54140R
英文名稱 NRF1 Recombinant Rabbit mAb
中文名稱 核呼吸因子-1重組兔單抗
別    名 erythroid derived 2; like 1; Locus control region factor 1; Locus control region-factor 1; NF-E2-related factor 1; NF2L1_HUMAN; NFE2 related factor 1; NFE2-related factor 1; NFE2L1; NRF1; NRF-1; NRF 1; Nuclear factor; Nuclear factor erythroid 2-related factor 1; Nuclear factor erythroid derived 2 like 1; TCF-11; TCF11; Transcription factor 11; Transcription factor HBZ17; Transcription factor LCR F1; Transcription factor LCR-F1.  
研究領域 染色質(zhì)和核信號  轉(zhuǎn)錄調(diào)節(jié)因子  合成與降解  線粒體  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 6G5
交叉反應 Human,Mouse,Rat
產(chǎn)品應用 WB=1:500,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55 kDa
檢測分子量
細胞定位 細胞核 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NRF1 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Function:
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Subunit:
Homodimer. Binds DNA as a dimer. Interacts with PPRC1.

Subcellular Location:
Nucleus

Tissue Specificity:
Ubiquitously expressed with strongest expression in skeletal muscle.

Post-translational modifications:
Phosphorylation enhances DNA binding.

Similarity:
Belongs to the NRF1/Ewg family.

SWISS:
Q16656

Gene ID:
4899

Database links:

Entrez Gene: 4899 Human

Omim: 600879 Human

SwissProt: Q16656 Human

Unigene: 654363 Human



產(chǎn)品圖片
Sample: Lane 1: Hela cell lysate Primary: Anti-NRF1 (bsm-54140R) at 1:1000 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 55 kD Observed band size: 65 kD
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human thyroid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (bsm-54140R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NRF1) monoclonal Antibody, Unconjugated (bsm-54140R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NRF1) monoclonal Antibody, Unconjugated (bsm-54140R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NRF1) monoclonal Antibody, Unconjugated (bsm-54140R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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