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TGF alpha Recombinant Rabbit mAb (bsm-54286R)  
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產品編號 bsm-54286R
英文名稱 TGF alpha Recombinant Rabbit mAb
中文名稱 轉移生長因子α抗體
別    名 EGF like TGF; ETGF; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1; TGFA_HUMAN; TGFα; TGF-α; TGF α.  
研究領域 腫瘤  心血管  信號轉導  生長因子和激素  細胞因子  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號
交叉反應 Human,Rat
產品應用 WB=1:200-1000,IHC-P=1:50-500,IHC-F=1:400-800,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 17 kDa
檢測分子量
細胞定位 細胞膜 細胞外基質 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human TGF alpha 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Function:
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar (By similarity). Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro).

Subunit:
Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2.

Subcellular Location:
Transforming growth factor alpha: Secreted, extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.

Similarity:
Contains 1 EGF-like domain.

SWISS:
P01135

Gene ID:
7039

Database links:

Entrez Gene: 403431 Dog

Entrez Gene: 7039 Human

Entrez Gene: 21802 Mouse

Entrez Gene: 24827 Rat

Omim: 190170 Human

SwissProt: P01135 Human

SwissProt: P48030 Mouse

SwissProt: P01134 Rat

Unigene: 170009 Human

Unigene: 137222 Mouse

Unigene: 9952 Rat



產品圖片
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
ICC staining TGF alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TGF alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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