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APG4B(Acetyl K39) Rabbit pAb (bs-2844R)  
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產品編號 bs-2844R
英文名稱 APG4B(Acetyl K39) Rabbit pAb
中文名稱 乙酰化自噬相關蛋白4B抗體
別    名 APG4B(Ac-Lys39); APG4B(Acetyl Lys39); APG4B(Ac-K39); APG4B; ATG4 autophagy related 4 homolog B(S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353br; ATG4B_HUMAN.  
產品類型 乙酰化抗體 
研究領域 腫瘤  細胞生物  信號轉導  細胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Pig)
產品應用 IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 44 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human APG4B(Acetyl K39) 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 ATG4B is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Function:
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Subcellular Location:
Cytoplasm (Probable).

Tissue Specificity:
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Similarity:
Belongs to the peptidase C54 family.

SWISS:
Q9Y4P1

Gene ID:
23192

Database links:

Entrez Gene: 23192 Human

Entrez Gene: 66615 Mouse

Entrez Gene: 316640 Rat

Omim: 611338 Human

SwissProt: Q9Y4P1 Human

SwissProt: Q8BGE6 Mouse

SwissProt: Q62625 Rat

Unigene: 283610 Human

Unigene: 29087 Mouse

Unigene: 163086 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2844R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2844R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2844R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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