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IL-6R alpha Rabbit pAb (bs-43015R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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產品編號 bs-43015R
英文名稱 IL-6R alpha Rabbit pAb
中文名稱 白細胞介素6受體α/IL-6Rα抗體
別    名 IL6R; CD 126; CD126; CD126 antigen; gp80; IL 6R 1; IL 6R alpha; IL 6R; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; Il6r; IL6RA; IL6RA_HUMAN; Interleukin 6 receptor alpha chain; Interleukin 6 receptor; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80;  
研究領域 細胞生物  免疫學  細胞膜受體  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50 kDa
檢測分子量
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human IL-6R alpha: 20-365/468 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].

Function:
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.

Subunit:
Hexamer of two molecules each of IL6, IL6R and IL6ST.

Subcellular Location:
Isoform 1: Basolateral cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Tissue Specificity:
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

Post-translational modifications:
A short soluble form may also be released from the membrane by proteolysis.

Similarity:
Belongs to the type I cytokine receptor family.
Type 3subfamily. Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

SWISS:
P08887

Gene ID:
3570

Database links:

Entrez Gene: 3570 Human

Entrez Gene: 16194 Mouse

SwissProt: P08887 Human

SwissProt: P22272 Mouse

SwissProt: P22273 Rat

Unigene: 2856 Mouse



產品圖片
Sample: Lane 1:Recombinant Human IL-6R alpha Protein at 50ng Lane 2:Recombinant Human IL-6R alpha Protein at 500ng Primary: Rabbit Anti-IL-6R alpha Antibody at 1/1000 dilution (Cat.bsm-43015R) Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: 58-75kD
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (bs-43015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-6R alpha) Polyclonal Antibody, Unconjugated (bs-43015R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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