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IFN-gamma Rabbit pAb (bs-41061R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產品編號 bs-41061R
英文名稱 IFN-gamma Rabbit pAb
中文名稱 干擾素-γ/IFN-γ抗體
別    名 IFNG; IFG; IFI; IFN gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.  
研究領域 腫瘤  免疫學  信號轉導  干擾素  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 Flow-Cyt=1ug/Test,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 18 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinded mouse IFN-gamma protein 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.

Function:
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

Tissue Specificity:
Released primarily from activated T lymphocytes.

Post-translational modifications:
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

DISEASE:
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

Similarity:
Belongs to the type II (or gamma) interferon family.

SWISS:
P01580

Gene ID:
15978

Database links:

Entrez Gene: 3458 Human

Entrez Gene: 15978 Mouse

Entrez Gene: 360697 Rat

Omim: 147570 Human

SwissProt: P01579 Human

SwissProt: P01580 Mouse

Unigene: 856 Human

Unigene: 240327 Mouse




產品圖片
4% Paraformaldehyde-fixed Jurkat(Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 μg/mL, 6 h), BFA (5 μg/ml, last 5 h))(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IFN gamma) polyclonal Antibody, unconjugated (bs-41061R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Jurkat(Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 μg/mL, 6 h), BFA (5 μg/ml, last 5 h)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-IFN gamma antibody (bs-41061R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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