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Azurocidin/HBP Mouse mAb (bsm-41703M)  
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產品編號 bsm-41703M
英文名稱 Azurocidin/HBP Mouse mAb
中文名稱 天青素/肝素結合蛋白單克隆抗體
別    名 Azu 1; Azu1; CAP37; CAP7_HUMAN; cationic antimicrobial protein 37; Cationic antimicrobial protein CAP37; HBP; Heparin binding protein; Heparin-binding protein; HUMAZUR.  肝素結合蛋白; 陽離子抗菌蛋白37; 天青殺素;
研究領域 心血管  細胞生物  免疫學  細菌及病毒  趨化因子  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 3G8
交叉反應 Human
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 24 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
免 疫 原 Recombinant Human Azurocidin Protein: 20-250/251 
亞    型 IgG1
純化方法 affinity purified by Protein A
緩 沖 液 0.01M PBS (pH7.4).
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.

Function:
This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.

Subunit:
Monomer.

Subcellular Location:
Cytoplasmic granule. Note=Cytoplasmic granules of neutrophils.
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.

Similarity:
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.

SWISS:
P20160

Gene ID:
566

Database links:

Entrez Gene: 566 Human

Omim: 162815 Human

SwissProt: P20160 Human

Unigene: 72885 Human



產品圖片
Sample: Lane 1:Recombinant Human Azurocidin Protein at 100ng Primary: Mouse Anti-Azurocidin Monoclonal Antibody at 1/1000 dilution (Cat.bsm-41703M) Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28.6kD Observed band size: 38-42kD
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (bsm-41703M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (bsm-41703M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (bsm-41703M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (bsm-41703M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Acute choriitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (bsm-41703M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human Azurocidin, His Tag (Cat. bs-43022P) at 2 μg/mL (100 μL/well) can bind Anti-HumanAzurocidin monoclonal antibody, the minimum detection concentration is 4ng/mL
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