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CK-7 Recombinant Rabbit mAb (bsm-60305R)  
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產(chǎn)品編號 bsm-60305R
英文名稱 CK-7 Recombinant Rabbit mAb
中文名稱 細胞角蛋白7重組兔單抗
別    名 Cytokeratin 7; Cytokeratin-7; CK 7; CK-7; K7; Keratin 7; Keratin, type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN.  
研究領(lǐng)域 信號轉(zhuǎn)導(dǎo)  b-淋巴細胞  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 C2G6
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:200-1:1000,IHC-F=1:50-100,IF=1:50-100,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 54 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CK-7 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

SWISS:
P08729

Gene ID:
3855

Database links:

Entrez Gene: 3855 Human

SwissProt: P08729 Human



產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa, MCF-7, A549 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 51 kDa Observed MW: 50 kDa
Tissue: Human ovarian carcinoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60305R
Tissue: Human breast cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60305R
Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60305R
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