| 產品編號 | bs-25282R |
| 英文名稱 | Hyaluronidase-1 Rabbit pAb |
| 中文名稱 | 透明質酸酶/玻璃酸酶抗體 |
| 別 名 | Hyaluronidase-1; HYAL1_HUMAN; Hyal-1; Hyaluronoglucosaminidase-1; Lung carcinoma protein 1 (LuCa-1); LUCA1; EC:3.2.1.35. |
| 研究領域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hyaluronidase-1: 161-260/435 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis. SWISS: Q12794 Gene ID: 3373 Database links: Entrez Gene: 3373 Human Entrez Gene: 15586 Mouse Omim: 607071 Human SwissProt: Q12794 Human SwissProt: Q91ZJ9 Mouse Unigene: 75619 Human Unigene: 10305 Mouse Unigene: 475658 Mouse Unigene: 124438 Rat
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| 產品圖片 |
Sample:
Lane 1: Rat Liver tissue lysates
Lane 2: Rat Spleen tissue lysates
Primary: Anti-Hyaluronidase-1 (bs-25282R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
